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The proliferation inhibiting and apoptosis inducing effects of chlorhexidine and essential oils mouthwashes on fibroblast cell line : In vitro study / Shaimaa Ali Hamouda ; Supervised Naglaa Elhossary , Nermine Raouf Amin

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Shaimaa Ali Hamouda , 2018Description: 112 P. : charts , facsimiles ; 25cmOther title:
  • التأثيرالمثبط للتكاثر والمحفزللموت المبرمج للكلورهيكسيدين والزيوت العطرية في خط الخلية الليفية : دراسة معملية [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology Summary: Objectives: This in vitro study evaluated the effect of Essential oils (EOs), Essential oils with sodium fluoride (EOF) and Chlorhexidine (CHX) mouth washes on viability and apoptosis of fibroblast cell line. Material and Methods: Baby hamster kidney -21 (BHK-21) fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium (EMEM) containing, EOs, EOF and CHX with different doses and various exposure times. Control cells received an equal volume of EMEM without adding mouth rinses. Cell viability and apoptosis were evaluated using MTT assay and RT-PCR. Results: The EOs, EOF and CHX mouthwashes inhibited fibroblast cell viability in a dose and time dependant manner. Conclusions: CHX mouthwash was the most cytotoxic to fibroblasts as compared to EOs and EOF. Moreover, adding fluoride to EOs in low concentrations didn`t worsen the adverse effects of EOs on cell viability and apoptosis induction
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.14.Ph.D.2018.Sh.P (Browse shelf(Opens below)) Not for loan 01010110077935000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.14.Ph.D.2018.Sh.P (Browse shelf(Opens below)) 77935.CD Not for loan 01020110077935000

Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

Objectives: This in vitro study evaluated the effect of Essential oils (EOs), Essential oils with sodium fluoride (EOF) and Chlorhexidine (CHX) mouth washes on viability and apoptosis of fibroblast cell line. Material and Methods: Baby hamster kidney -21 (BHK-21) fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium (EMEM) containing, EOs, EOF and CHX with different doses and various exposure times. Control cells received an equal volume of EMEM without adding mouth rinses. Cell viability and apoptosis were evaluated using MTT assay and RT-PCR. Results: The EOs, EOF and CHX mouthwashes inhibited fibroblast cell viability in a dose and time dependant manner. Conclusions: CHX mouthwash was the most cytotoxic to fibroblasts as compared to EOs and EOF. Moreover, adding fluoride to EOs in low concentrations didn`t worsen the adverse effects of EOs on cell viability and apoptosis induction

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