Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

A swab samples were collected from different suspected sources of bacterial contamination in El-Monieb slaughterhouse in Giza Governorate. The results showed that out of the 345 samples, S. aureus was detected in 40.6%.The mean count between the different sources of contamination was 4.9 ± 1.5 log10CFU/ cm2. The antimicrobial susceptibility pattern showed that all isolates of S. aureushave antimicrobial resistance. By PCR amplification, mecA was detected confirming the isolation of methicillin resistant S. aureusin percentage 13%. E. faecaliswere isolated by 35.4% with an average mean count 5.73 ± 1.7 log10 CFU/ cm2. The antimicrobial susceptibility pattern showed that all isolates were also resistant. The vanA genewas detected confirming the isolation of vancomycin resistant E. faecalisin 21.3%. Salmonella was isolated in 1.4%. There weredifferent sources of contaminationin the slaughterhouse before, during and after the slaughtering process which could threaten public health and play a potential role in food safety. Different disinfectants were tested by spraying method to decrease the bacterial load on the surface of the utensils used in slaughtering, floor and walls inside the slaughterhouse.H2O2plus peracetic acid was found to be the most effective disinfectant

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