Investigation of certain Egyptian plants on the apoptosis of cancer cells / Sherif Mahmoud Mohamed Afifi ; Supervised Essam Abdelsattar , Azza Ramy Abdelmonem , Mohamed Elamir

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

Soybean (Glycine max L.) is an important worldwide crop, mainly used as raw material for oil production, and the residue is mainly used as feedstuff. The medicinal functions of consuming soybean have been shown to be effective against number of chronic diseases such as cancer, heart disease, osteoporosis and menopausal symptoms. In recent years there has been a gradual revival of interest in the use of medicinal plants because herbal medicine have been reported safe and less or without any adverse effect especially when compared with synthetic drugs.The present study includes three parts: DNA fingerprinting, phytochemical study and biological study. The Random Amplified Polymorphic DNA (RAPD)analysis revealed 72 total scorable bands in the range of 230-1700 bp. with high level of polymorphism (75%).The number of polymorphic bands ranged between 2 and 8 with polymorphism range from 50% to 88.9%.These results implied that these primers have good potential for identifying DNA polymorphisms and analyzing the genetic relationship among soybean cultivars. For the phytochemical analyses of soybean, HPLC-ESI-MS analysis in both positive and negative ionization modes were utilized. Twenty-nine metabolites were tentatively characterized in the three studied cultivars as twelve soyasaponins, twelve isoflavones and four phenolic acids, in addition to one anthocyanin

Issued also as CD