Physiological studies on jojoba plant [Simmondsia chinensis (link) schneider] using tissue culture techniques / Bassant Mubarak Mohamed Hamdy ; Supervised Osama Mohamed Elshihy , Ahmed Shawky Ibrahim

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Bassant Mubarak Mohamed Hamdy

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TextLanguage: English Publication details: Cairo : Bassant Mubarak Mohamed Hamdy , 2018Description: 84 P. : charts , facsimiles ; 25cmOther title:

دراسات فسيولوجية على نبات الجوجوبا باستخدام تقنيات زراعة الأنسجة [Added title page title]

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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany Summary: The current work was conducted to improve the regeneration capacity of jojoba plants from callus derived from female leaf explants. Therefore, an improved culture medium was developed and used in the main experiments of regeneration via induction of adventitious shoot buds. Thus, leaf explants of female jojoba plants were cultured on an improved media for regeneration of jojoba plants; callus induction medium (CIM), bud formation medium (BFM), shoot development medium (SDM) and root development medium (RDM), containing various concentrations of plant growth regulators, auxin (2,4-D, Dicamba, NAA or IBA) either alone or in combination with cytokinin (BA or TDZ). The obtained results revealed that, the use of the combination between Dicamba (1.5 mg/l) and TDZ (0.75 mg/l) in CIM produced the highest percentage of callus induction (98.65 %). In addition, the use of TDZ (2.5 mg/l) in combination with Dicamba (0.5 mg/l) in BFM produced the highest percentage of shoot regeneration (99.65%) with the highest average number of the produced shoots/callus (23.18)

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.05.M.Sc.2018.Ba.P (Browse shelf(Opens below))		Not for loan		01010110078557000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.05.M.Sc.2018.Ba.P (Browse shelf(Opens below))	78557.CD	Not for loan		01020110078557000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany

The current work was conducted to improve the regeneration capacity of jojoba plants from callus derived from female leaf explants. Therefore, an improved culture medium was developed and used in the main experiments of regeneration via induction of adventitious shoot buds. Thus, leaf explants of female jojoba plants were cultured on an improved media for regeneration of jojoba plants; callus induction medium (CIM), bud formation medium (BFM), shoot development medium (SDM) and root development medium (RDM), containing various concentrations of plant growth regulators, auxin (2,4-D, Dicamba, NAA or IBA) either alone or in combination with cytokinin (BA or TDZ). The obtained results revealed that, the use of the combination between Dicamba (1.5 mg/l) and TDZ (0.75 mg/l) in CIM produced the highest percentage of callus induction (98.65 %). In addition, the use of TDZ (2.5 mg/l) in combination with Dicamba (0.5 mg/l) in BFM produced the highest percentage of shoot regeneration (99.65%) with the highest average number of the produced shoots/callus (23.18)

Issued also as CD

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