Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

Liver fibrosis is a major health problem that can lead to liver cirrhosis and hepatic carcinoma. This study investigates the hepatoprotective effect of bone marrow mononuclear cells (BM-MNCs) transplantation, N-acetylcysteine (NAC) and Ü-lipoic acid (ALA) against hepatic toxicity and cytogenetical aberrations experimentally induced by carbon tetrachloride (CCl₄ in rats. Rats are administrated CCl4 (1mg/kg, i.p.) twice/week for 8 weeks showed elevation of liver enzymes ALT, AST in serum, liver tissue TNF-Ü, IL-6 inflammatory cytokines, while a reduction of albumin serum level and IL-10 anti-inflammatory cytokines, oxidative stress biomarkers as SOD, CAT, GPx, TAC liver tissue content accompanied by elevation of MDA liver content. In addition to cytogenetical aberration assessment via micronucleated polychromatic erythrocytes (MnPCEs) count, percent (%) of DNA damaged cells. Administration of BM-MNCs (1{u00D7}10⁶ in 0.1ml PBS, i.v.), NAC (300 mg/kg, p.o), ALA (100 mg/kg, p.o) alone and their combinations (BM-MNCs with NAC and BM-MNCs with ALA) decreased tissue content of TNF-Ü, IL-6, MDA, percentage of DNA damaged cells and MnPCEs and increase others as GPx, CAT, SOD, TAC and IL-10. Also, these agents produced marked reduction of liver enzymes ALT, AST, serum albumin

Issued also as CD