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In vitro study of metformin effect on glypican3 and long non coding RNA gene expression in hepatocellular carcinoma cell line / Mona said Abdelghany Hassan ; Supervised Omayma Ali Elkholy , Radwa Mohamed Taha , Naglaa Fathy Mahmoud

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mona said Abdelghany Hassan , 2019Description: 131 P. : charts , facsimiles ; 25cmOther title:
  • دراسة لمعرفة تأثير المتفورمين علي التعبير الجيني للجلابكن 3 وحمض الريبونيوكليك الطويل الغير مشفر في خلايا الكبد السرطانية خارج جسم الانسان [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry Summary: Background: Hepatocellular carcinoma (HCC) has been one of the main reasons of cancer-associated mortality. Despite the recent advances in the clinical management of liver cancer, the long-term prognosis of HCC is still dismal. Developing new treatment strategies for HCC could be one of the pressing needs. The biguanide Metformin is a first-line drug for treatment type II diabetes mellitus. In addition it is considered a promising prospect in lowering risk, treatment and prognosis of HCC, but its precise antitumor mechanisms remain unclear. Aim: This study aimed to assess the dose and duration effects of metformin on cancer cell viability and levels of gene expression of (glypican3, LncRNA -AF085935, VEGF, P53 and NFmB). Methods: The study was conducted in vitro on HCC cell line, HepG2. It was treated by different concentrations of metformin (5, 10 and 20oM) at different durations (24, 48 and 72 h) versus untreated control groups. Cells viability were evaluated by MTT assay, while gene expression of interesting genes was assessed by quantitative real time PCR. Finally we statically analyzed a correlation between glypican3 and lncRNA-AF085935, cell proliferation and each studied parameter i.e. Glypican3 and LncRNA-AF085935 expression among studied groups
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.03.M.Sc.2019.Mo.I (Browse shelf(Opens below)) Not for loan 01010110078774000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.03.M.Sc.2019.Mo.I (Browse shelf(Opens below)) 78774.CD Not for loan 01020110078774000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry

Background: Hepatocellular carcinoma (HCC) has been one of the main reasons of cancer-associated mortality. Despite the recent advances in the clinical management of liver cancer, the long-term prognosis of HCC is still dismal. Developing new treatment strategies for HCC could be one of the pressing needs. The biguanide Metformin is a first-line drug for treatment type II diabetes mellitus. In addition it is considered a promising prospect in lowering risk, treatment and prognosis of HCC, but its precise antitumor mechanisms remain unclear. Aim: This study aimed to assess the dose and duration effects of metformin on cancer cell viability and levels of gene expression of (glypican3, LncRNA -AF085935, VEGF, P53 and NFmB). Methods: The study was conducted in vitro on HCC cell line, HepG2. It was treated by different concentrations of metformin (5, 10 and 20oM) at different durations (24, 48 and 72 h) versus untreated control groups. Cells viability were evaluated by MTT assay, while gene expression of interesting genes was assessed by quantitative real time PCR. Finally we statically analyzed a correlation between glypican3 and lncRNA-AF085935, cell proliferation and each studied parameter i.e. Glypican3 and LncRNA-AF085935 expression among studied groups

Issued also as CD

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