Potentiality of some fungal cultures for production of bioactive secondary metabolites with antioxidant activity / Alaa Mohamed Saleh Saleh ; Supervised Abdelgawad M. Hashem , Omneya Mohamed Helmy , Heba A. Elrefai

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Alaa Mohamed Saleh Saleh

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TextLanguage: English Publication details: Cairo : Alaa Mohamed Saleh Saleh , 2019Description: 107 P. : charts ; 25cmOther title:

كفاءة بعض الفطريات لإنتاج مركبات أيضية ثانوية ذات فاعلية بيولوجية كمضادات للأكسدة [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology Summary: The fungi A.terreus MH205918 extract showed promising DPPH scavenging activity. Statistical optimization of scavenging activity and total phenolic content resulted in 1.17 fold and 2.37 fold increase compared to basal medium respectively. Butyrolactone I (BL-1) was isolated as major active compound. Total extract and BL-1 showed concentration dependent antioxidant activities in different assays. Molecular docking of BL-1 with the regulatory protein Keap1 showed good interaction. Total extract and BL-1 increased induction of NQO1 in (hepa1c1c7). The total fungal extract and BL-1 showed antimicrobial activity against G+ve and G-ve bacteria. BL-1 showed cytotoxicity against A549 cell line and no activity against normal cell line (BJ1). Two gene fragments, were amplified using PCR from A.terreus MH205918 isolated DNA. One fragment showed 98% similarity to the A.terreus (NIH2624) ATET- 05798 indicated as conserved hypothetical protein in the database while the other, accession number MG881210, showed 96.1 % similarity to the A.terreus (NIH2624) ATEG-00108 gene responsible for Glyoxalase I enzyme

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.08.06.Ph.D.2019.Al.P (Browse shelf(Opens below))		Not for loan		01010110078928000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.08.06.Ph.D.2019.Al.P (Browse shelf(Opens below))	78928.CD	Not for loan		01020110078928000

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

The fungi A.terreus MH205918 extract showed promising DPPH scavenging activity. Statistical optimization of scavenging activity and total phenolic content resulted in 1.17 fold and 2.37 fold increase compared to basal medium respectively. Butyrolactone I (BL-1) was isolated as major active compound. Total extract and BL-1 showed concentration dependent antioxidant activities in different assays. Molecular docking of BL-1 with the regulatory protein Keap1 showed good interaction. Total extract and BL-1 increased induction of NQO1 in (hepa1c1c7). The total fungal extract and BL-1 showed antimicrobial activity against G+ve and G-ve bacteria. BL-1 showed cytotoxicity against A549 cell line and no activity against normal cell line (BJ1). Two gene fragments, were amplified using PCR from A.terreus MH205918 isolated DNA. One fragment showed 98% similarity to the A.terreus (NIH2624) ATET- 05798 indicated as conserved hypothetical protein in the database while the other, accession number MG881210, showed 96.1 % similarity to the A.terreus (NIH2624) ATEG-00108 gene responsible for Glyoxalase I enzyme

Issued also as CD

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