Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

The objective of this study was to evaluate the protective and therapeutic effects of areal parts of Pulicaria crispa (Forssk.) ethanol extract against ethanol induced gastric ulcer in rats. Phytochemical screening of each extract was also done to identify the compounds responsible for protecting and healing the ulcer. Plant extract (500 mg/kg b.wt.) was orally administered daily for seven days prior or after ulceration with one oral dose of 1 ml ethanol on 24 hours empty stomach. Ranitidine (100 mg/kg b.wt.) was used as a reference drug. Rats were divided into nine groups. Group one served as control. Groups 2, 3 administered with plant extract and Ranitidine as a reference drug. Group 4 was ulcerative rats for one hour. Groups 5, 6 received plant extract or Ranitidine prior ulcer induction. Groups 7, 8 received plant extract or Ranitidine post ulcer induction. Group 9 was ulcerative stomach recovery group. The evaluation was carried out through measuring ulcer indices; stomach acidity and volume as well as the lesion counts. Malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) as oxidative stress markers were evaluated. Certain marker enzymes for different cell organelles; succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glucose-6-phosphatase (G-6-Pase), acid phospatase (AP) and 5`-nucleotidase (5`NT) were also estimated. Inflammatory indices as interlukin-10 (IL- 10), intracellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor alpha (TNF-Ü) were also determined

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