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Recognition of two species of genus chlamydia derived from sheep, goats and cattle : Chlamydia abortus and Chlamydia pecorum / Ghada Sayed Abdelaziz ; Supervised Wagih Armanious Gad , Ahmed Samir Mohamed , Hadia Abdelrehim Ali

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ghada Sayed Abdelaziz , 2019Description: 148 P. : charts , facsimiles ; 25cmOther title:
  • تعرف على نوعين من جنس الكلاميديا مستمدين من الاغنام والماعزوالابقار : كلاميديا ابورتس وكلاميديا بيكورم [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: The chlamydiae are a diverse group of obligate intracellular Gram-negative bacteria that are known to infect a wide variety of host species. The present study wasconducted to detect the incidence of Chlamydia abortus and Chlamydia pecorumamong cattle, sheep and goats. It was carried out on two groups of animals: the first one consists of apparently healthy cattle (n=35), sheep (n=8) and goats (n=23). The second one consists of diseased cattle (n=14), sheep (n=21) and goats (n=18) with different clinical findings such as: respiratory disorders, keratoconjunctivitis, vaginitis, abortion and diarrheaand the source of specimens were fecal (n=40), nasal (n=30), ocular (n=3) and vaginal samples (n=18). Moreover samples from internal organs (n=10) and sera (n=65) were also collected.Criteria of samples examination for detection of Chlamydia abortus and Chlamydia pecorum were as follow: CFT was used for determination of Chlamydia abortus and Chlamydia pecorum antibodies and antigen, Indirect Immunofluorescence test was used to confirm the presence of inclusion bodies of Chlamydia spp., Chlamydia abortus and Chlamydia pecorum were isolated by inoculation in 7-day old ECE via yolk sac route, TEM was used to confirm the presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum in infected yolk sac andThe collected internal organs from animals were stained by Giemsa stain for detection of inclusion bodies of Chlamydia abortus and Chlamydia pecorum. Results of CFT reveal that serum samples of cattle, sheep and goats were positive for Chlamydia spp. antibodies in a rate of 85%, fecal samples in cattle, sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 77.5%, while vaginal swabs in sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 70%, whereasnasal swabs in cattle and sheep showed positive results for Chlamydia spp. antigen in a rate of 77% and all ocular swabs in sheep showed positive results for Chlamydia spp. antigen
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2019.Gh.R (Browse shelf(Opens below)) Not for loan 01010110080069000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2019.Gh.R (Browse shelf(Opens below)) 80069.CD Not for loan 01020110080069000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

The chlamydiae are a diverse group of obligate intracellular Gram-negative bacteria that are known to infect a wide variety of host species. The present study wasconducted to detect the incidence of Chlamydia abortus and Chlamydia pecorumamong cattle, sheep and goats. It was carried out on two groups of animals: the first one consists of apparently healthy cattle (n=35), sheep (n=8) and goats (n=23). The second one consists of diseased cattle (n=14), sheep (n=21) and goats (n=18) with different clinical findings such as: respiratory disorders, keratoconjunctivitis, vaginitis, abortion and diarrheaand the source of specimens were fecal (n=40), nasal (n=30), ocular (n=3) and vaginal samples (n=18). Moreover samples from internal organs (n=10) and sera (n=65) were also collected.Criteria of samples examination for detection of Chlamydia abortus and Chlamydia pecorum were as follow: CFT was used for determination of Chlamydia abortus and Chlamydia pecorum antibodies and antigen, Indirect Immunofluorescence test was used to confirm the presence of inclusion bodies of Chlamydia spp., Chlamydia abortus and Chlamydia pecorum were isolated by inoculation in 7-day old ECE via yolk sac route, TEM was used to confirm the presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum in infected yolk sac andThe collected internal organs from animals were stained by Giemsa stain for detection of inclusion bodies of Chlamydia abortus and Chlamydia pecorum. Results of CFT reveal that serum samples of cattle, sheep and goats were positive for Chlamydia spp. antibodies in a rate of 85%, fecal samples in cattle, sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 77.5%, while vaginal swabs in sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 70%, whereasnasal swabs in cattle and sheep showed positive results for Chlamydia spp. antigen in a rate of 77% and all ocular swabs in sheep showed positive results for Chlamydia spp. antigen

Issued also as CD

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