Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Ageing is an unavoidable, universal, biological phenomenon affecting all organisms that involves variable declines of individuals motor and memory capabilities. Recently, the search for effective natural compounds with anti-ageing activity has been intensified. We aimed to investigate the potential ameliorating effects of Curcumin C3 Complex, Astragalus Membranaceus and Blueberry on certain age-related biochemical and molecular alterations inrat liver. The study included five groups of rats; group 1, adult control group aged 5 months-old, and groups (2-5) aged 12 months-old. Group 2,aged control group; was kept without any treatment until the age of 17 months. Groups (3-5) receiveddaily by oral gavage for 5 months the following supplements: Curcumin C3 Complex (110mg/kg), Astragalus Membranaceus(100mg/kg) and Blueberry (100mg/kg)respectively.At the end of the experiment,animals were sacrificed and serum Interleukin-6 (IL-6)was determined. Liver was isolated for estimation of malondialdehyde (MDA),8-hydroxy-2{u2032}-deoxyguanosine (8-OHdG),total antioxidant capacity (TAC) and nitric oxide (NO), in addition to IL-6 and telomerase reverse transcriptase (TERT) gene expressions. Hepatic telomere length and lipofuscin contents were also investigated

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