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T-cell polarization in breast cancer : A potential role of syndecan-1 / Moshira Ezzat Mostafa Mohamed ; Supervised Ahmed M. F Afifi , Sherif Abdelaziz Ibrahim , Mohamed Elsayed Elshinawi

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Moshira Ezzat Mostafa Mohamed , 2019Description: 174 P. : charts , facsimiles ; 25cmOther title:
  • إستقطاب الخلايا التائية فى سرطان الثدى : الدور المحتمل للسينديكان - 1 [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: This study aimed to identify the immunomodulatory role of tumor Syndecan-1 (Sdc-1) in the polarization of T helper (Th) subsets isolated from the tumor microenvironment of inflammatory breast cancer (IBC) and non-IBC patients. Two models of co-cultures (direct & indirect) were established with Sdc-1-silenced SUM-149 cells and axillary Th cells of IBC and non-IBC patients. Employing flow cytometry, the frequencies of Th₁, Th₂, Th₁₇, and Treg subsets for basal and cocultured cells were assessed. The data revealed a lower basal frequency of Th₁and Th₂ subsets in IBC than non-IBC. Sdc-1-silenced SUM-149 cells significantly enhanced the polarization of Th₁₇ and Treg subsets of non-IBC under both direct and indirect conditions. Interestingly, qPCR showed a negative correlation between Sdc- 1 and each of IL-4, IL-17, and Foxp3 mRNA expression in carcinoma tissues of IBC that was reversed in non-IBC. Mechanistically, Sdc-1 knockdown in SUM-149 cells promoted Th₁₇ cell expansion via up-regulation of IL-23 and the Notch ligand DLL4. Overall, this study suggests an immunoregulatory role of tumor Sdc-1 expression in Th cell polarization that may have therapeutic implications for breast cancer
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2019.Mo.T (Browse shelf(Opens below)) Not for loan 01010110080137000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2019.Mo.T (Browse shelf(Opens below)) 80137.CD Not for loan 01020110080137000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

This study aimed to identify the immunomodulatory role of tumor Syndecan-1 (Sdc-1) in the polarization of T helper (Th) subsets isolated from the tumor microenvironment of inflammatory breast cancer (IBC) and non-IBC patients. Two models of co-cultures (direct & indirect) were established with Sdc-1-silenced SUM-149 cells and axillary Th cells of IBC and non-IBC patients. Employing flow cytometry, the frequencies of Th₁, Th₂, Th₁₇, and Treg subsets for basal and cocultured cells were assessed. The data revealed a lower basal frequency of Th₁and Th₂ subsets in IBC than non-IBC. Sdc-1-silenced SUM-149 cells significantly enhanced the polarization of Th₁₇ and Treg subsets of non-IBC under both direct and indirect conditions. Interestingly, qPCR showed a negative correlation between Sdc- 1 and each of IL-4, IL-17, and Foxp3 mRNA expression in carcinoma tissues of IBC that was reversed in non-IBC. Mechanistically, Sdc-1 knockdown in SUM-149 cells promoted Th₁₇ cell expansion via up-regulation of IL-23 and the Notch ligand DLL4. Overall, this study suggests an immunoregulatory role of tumor Sdc-1 expression in Th cell polarization that may have therapeutic implications for breast cancer

Issued also as CD

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