Assessment of vitamin D status in Egyptian patients with Hashimoto`s thyroiditis / Hany William Zaki Hanna ; Supervised Hazem Elsayed Abou Youssef , Hemmat Elewa Elhaddad , Cristiano Rizzo

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Hashimoto's thyroiditis is considered the most common autoimmune disease and the predominant cause of hypothyroidism in iodine sufficient countries. Twenty five hydroxy D3/D2 status was found incriminated in many adverse health states especially autoimmune diseases. This could be attributed to its serum level deficiency and polymorphisms of vitamin D receptor (VDR). Aim: This study aimed to assess and compare serum (25 (OH) D3/D2) level in Egyptian adult patients with Hashimoto's thyroiditis and non - Hashimoto's hypothyroidism and to test the hypothesis linking vitamin D receptor gene (FokI and BsmI) polymorphisms with Hashimoto's thyroiditis. Subjects: The current study included 160 subjects, 112 patients with Hashimoto's thyroiditis and 48 hypothyroid non-Hashimoto's controls. They were diagnosed based on anti-TPO Ab and or anti-TG Ab results. Methods: All included patients were subjected to full history taking, clinical examination, thyroid ultrasound examination, panel of assay (TSH, f.T3, f.T4, anti-TPO Ab, anti-TG Ab, calcium, alkaline phosphatase and phosphate). Serum 25(OH) D3/D2 was assayed for all subjects using HPLC-UV in-house method. Vitamin D receptor gene polymorphism (FokI and BsmI) using real-time PCR were done for 118 patients (70 Hashimoto's patients randomly chosen and 48 control subjects)

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