A comparative pharmacognostical study of certain jasminum species / Fatma Alzahraa Mokhtar Ali ; Supervised Seham Salah Eldin Elhawary , Hala M. Elhefnawy , Samir M. Osman

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

Jasminum is a genus of flowering climbing shrubs and vines (family Oleaceae). Genus Jasminum composed of over 2000 plants distributed all over the world. They are native to Eurasia, India, and the Mediterranian region. Jasminum. azoricum L., Jasminum humile L., Jasminum multiflorum Burm.f.., Jasminum officinale L., Jasminum sambac L. (Arabian nights) and Jasminum sambac L. (Grand Duke of Tuscany) were collected from AL-Keram farms, Al-Beheira Government. The plants; were subjected to DNA fingerprinting using SCOT and ISSR primers for genetic characterization. The study was performed on biologically guided bases, so four in-vitro biological studies were performed; antioxidant, antimicrobial, cytotoxic effect on three cell lines and antihepatic C virus. The comparative biological studies showed that J. multiflorum leaves have higher antioxidant effects by DPPH assay, while J. officinale and J. sambac (G) flower extracts showed more antimicrobial effects than other species and cultivars. The cytotoxicity study showed that all the species possessed cytotoxic effects in different degrees, the higher cytotoxic effects observed in J. humile and J. sambac. In-vitro HCV activity was performed in addition to cell genes analysis by western blot technique, proved that J. multiflorum leaves and flowers can decrease the viral load and inhibit its replication by 85-88%. They inhibit viral replication by affecting NS5A protein and inhibit viral penetration and expansion of infection by affecting the EPAH2 gene

Issued also as CD