Bacterial and fungal profile of keratitis among patients in research institute of ophthalmology / Amal Ibrahim Abdelgayed Abouzeid ; Supervised Somaia Abdellatif Eissa , Alaa Mohamed Reda Awad , Amal Elsaed Aboelnoor

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Infectious keratitis is an ocular emergency that requires prompt and specific management to preserve ocular integrity. It is infection of the cornea by infective organisms like bacteria, fungi, virus or parasite. This study was carried out on 50 patients presented with infectious keratitis to the Cornea Outpatient Clinic of Research Institute of Ophthalmology from April 2017 to December 2017.Their demographic characteristics and risk factors were recorded. After detailed ocular examinations, ophthalmologist collected a corneal sample after taking the patient{u2019}s consent.Corneal specimens were processed for direct gram stain. The sample was streaked on blood agar, chocolate agar, MacConkey agar and Sabouraud dextrose agar (SDA) in multiple C or linear shaped streaks.The bacterial isolates were identified based on colony morphology, microscopic examination and biochemical reactions followed by antimicrobial sensitivity according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The fungal growth was identified by its colony morphology, pigment production and lacto phenol cotton blue mount examination.Most of the cases (58%) were in the age group between 41-60. Males represented 54% while female represented 46% of cases. Sixty two percent of cases lived in rural areas while 38% in urban areas. Regarding patients occupation; housewives represented 38%, farmers 30%. Trauma was the most common predisposing factor observed in 15/50 (30%) of the patients

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