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Molecular and epidemiological investigation of lymphocystis disease virus in Egyptian marine aquaculture / Mohamed Shawky Helmy Abdalhamed Khalifa ; Supervised Ausama Abdelraouf Abdelmoneim Yousif , Mohamed Abdelaziz Ahmed , Mohamed Faisal Abdelkariem

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mohamed Shawky Helmy Abdalhamed Khalifa , 2019Description: 89 P. : facsimiles ; 25cmOther title:
  • الاستقصاء الجزيئى والوبائى عن فيروس مرض الليمفوسيستس فى مزارع اسماك المياه المالحة المصرية [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: Egyptian marine aquacultures are expanding. Growth in the aquaculture industry is expected because of the abundance of water bodies, the demand for high quality inexpensive protein, and the need for economic structures to support the expanding population. Aquaculture conditions are often conducive to the spread of diseases; pathogens, including viruses, that would normally exist in relatively low levels in wild fish populations are problematic in densely packed aquaculture. There is little information about pathogenic fish viruses in Egypt even though members of twelve virus families have been identified in wild and cultured fish species worldwide. Molecular diagnostic assays are preferred in epidemiological investigations because they are rapid, sensitive, specific, and provide temporal and spatial epidemiological information when properly diploid. Virus isolation, on the other hand, is difficult, time-consuming and often ineffective for detection of viruses. Development of diagnostic assays for fish viruses requires the presence of proper positive control, which is not readily available in Egypt. One of the objectives of this study was to develop a synthetic stable positive control for five viral pathogens that threaten aquaculture species; Lymphocystis Disease Virus (LCDV) was one of selected viruses. A fragment of the major capsid protein (MCP) gene of LCDV was targeted for synthesis and cloning. A diagnostic assay was then developed, its detection sensitivity was estimated, and used to investigate an outbreak of LCDV in a major marine aquaculture facility. The findings of this investigation lead to the pathological, ultrastructural, and phylogenetic characterization of the first known genotype I LCDV infection in cultured gilt-head seabream worldwide
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2019.Mo.M (Browse shelf(Opens below)) Not for loan 01010110080793000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2019.Mo.M (Browse shelf(Opens below)) 80793.CD Not for loan 01020110080793000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

Egyptian marine aquacultures are expanding. Growth in the aquaculture industry is expected because of the abundance of water bodies, the demand for high quality inexpensive protein, and the need for economic structures to support the expanding population. Aquaculture conditions are often conducive to the spread of diseases; pathogens, including viruses, that would normally exist in relatively low levels in wild fish populations are problematic in densely packed aquaculture. There is little information about pathogenic fish viruses in Egypt even though members of twelve virus families have been identified in wild and cultured fish species worldwide. Molecular diagnostic assays are preferred in epidemiological investigations because they are rapid, sensitive, specific, and provide temporal and spatial epidemiological information when properly diploid. Virus isolation, on the other hand, is difficult, time-consuming and often ineffective for detection of viruses. Development of diagnostic assays for fish viruses requires the presence of proper positive control, which is not readily available in Egypt. One of the objectives of this study was to develop a synthetic stable positive control for five viral pathogens that threaten aquaculture species; Lymphocystis Disease Virus (LCDV) was one of selected viruses. A fragment of the major capsid protein (MCP) gene of LCDV was targeted for synthesis and cloning. A diagnostic assay was then developed, its detection sensitivity was estimated, and used to investigate an outbreak of LCDV in a major marine aquaculture facility. The findings of this investigation lead to the pathological, ultrastructural, and phylogenetic characterization of the first known genotype I LCDV infection in cultured gilt-head seabream worldwide

Issued also as CD

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