Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

Microalgae are a potential feedstock for a vast group of products and renewable material for different applications in biotechnology. Anabaena oryzae, Chlorella vulgaris and a mixture of both were successfully cultivated in different concentrations of sweet whey, sewage and poultry waste extract as a promising alternative media which enhanced microalgal lipid accumulation. Their molecular identification was carried out and recorded accession no. in Gene Bank was MN658404 for Anabaena and MN658403 for Chlorella. 50%, whey, 75% sewage and 5 g/L poultry waste extract gave the maximum chl. a, dry weight and lipid content of the tested cultures compared with control (BBM) cultures. Maximum biomass productivity (51.86 mg/L/day) and lipid productivity (10.53 mg/L/day) were recorded by Chlorella culture with 5 g/L poultry waste extract. Role of microalgae in bioremediation of the three used wastes was determined, where reduction in all tested parameters (TDS, TSS, COD, BOD, NH4, NO3 TN and P) were recorded, promising clean environment and decreasing pollution. Light intensity of 81 oE m-2s-1, pH of 7.3 and 25 {u00B0}C were the most suitable for growth and lipid accumulation in the tested microalgae. Moreover the best used alternative medium was poultry waste extract 5g/L. Microwave pretreatment of harvested cells, chloroform/methanol 2:1 v/v and 5 hr. were the most effective for lipid extraction from the cells of the tested microalgae. Esterification and GC-Ms analysis for fatty acid profiles were estimated

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