Isolation and molecular characterization of salivary glands gene from Culex pipiens mosquito : Diptera;Culicidae / Soad Ali Hassan Mohamed ; Supervised Ismail Ezzet Ismail , Alaa Edeen Seufi Mohammed

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Entomology

In the current study, we investigated the expression profile of salivary glands from the mosquito culex pipiens (Diptera: culicidae), a principal vector of west Nile virus. Our analyses using Native and SDS-PAGE combined with differential display polymerase chain reaction were aimed to functional characterization of salivary molecules expressed in the saliva. To gain a better understanding into their roles during blood feeding. SG protein expression was compared among sugar-fed (SF), starved, unfed and blood-fed (BF) of the salivary glands of males and females of Cx. pipiens mosquito at different times of feeding habits. To defining whether diet (nectar, blood) influences the expression of Cx salivary gland-expressed genes in laboratory. Our results revealed that sugar feeding stages showed less polymorphism (in salivary gland proteins) than blood feeding stages. Starvation of males and females induced more polymorphic bands in salivary glands than in the case of unfed stage after emergence from pupa. The higher degree of polymorphism (in salivary gland proteins) is displayed in the case of blood feeding females. Sequencing of the eluted bands was performed and the generated sequences were blasted to three isoforms of Apyrase (blood fed female apyrase, sugar fed female apyrase and male apyrase) and Adenosine deaminase genes

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