Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Background/Aims: Fipronil (FPN) toxicity received substantial consideration as it can induce oxidative tissue damage and may be contemplated as apoptosis inducer. Our aim was to investigate the possible hepatic and neuro-protective roles of garlic or allopurinol against sub-acute toxicity of fipronil. Methods:Thirty-six mature male albino were randomly divided into six experimental groups as follows: the first group was saved as control (C) whereas the 2nd (G) and 3rd (A) were orally received 500 mg/kg aqueous garlic extract and 150 mg/L allopurinol in their drinking water, respectively.The 4th (F) one was orally received 13.277 mg/kg fipronil (1/10 LD50) while 5th (G+F) and 6th (A+F) groups were received the same doses of garlic and allopurinol, respectively, two hours before fipronil administration. At the end of the experiment, specimens of liver and brain were removed immediately from sacrificed animals and kept frozen at -80{u00B0}C for biochemical parameters assay. Other Liver and brain tissue pieces were used for histopathological examination, real time polymerase chain reaction (RT-PCR) and quantification of DNA fragmentation.Results: The results revealed that FPN motivated hepatic and brain oxidative damage which indicated as significant increase in malondialdehyde, protein carbonyl levels as well as enzymatic antioxidants activities (superoxide dismutase, catalase, glutathione peroxidase and xanthine oxidase), but glutathione-S-transferase was scored a significant decreased as compared to control

Issued also as CD