Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Analytical Chemistry

This work is concerned with synthesis and optimization of molecularly imprinted polymer specific for nadifloxacin. First, twelve molecularly imprinted polymers were synthesized and optimized using nadifloxacin as a template with different ratios of methacrylic acid and/or 4-vinyl pyridine as a functional monomers and ethylene glycol dimethacrylate as a cross-linker. The synthesized polymers that showed the best binding behavior were MIP₁(1:4:20) (template: methacrylic acid: ethylene glycol dimethacrylate) and MIP₂(1:2:2:20) (template: methacrylic acid: 4-vinyl pyridine: ethylene glycol dimethacrylate). Using further investigations, MIP₁showed higher binding capacity and selectivity than MIP₂. The solid phase extractionprocedures were optimized using MIP1 as a sorbent.The studied factors were: polymer weight, cartridge capacity, loading, washing and elution solvents, cartridge selectivity and cartridge reusability. The optimum procedures were using: 80 mg polymer, methanol as a loading solvent, water: acetonitrile (80:20; v/v) as a washing solvent and methanol: acetic acid (90:10; v/v) as elution solvent. Then, the optimized cartridge was used for specific extraction of nadifloxacin from real human plasma samples

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