Utilization of medicinal plants as a safe eco-friendly approach for phyto-parasitic nematode control / Abdullah Ali Abdelrahman ; Supervised Alsayed Abuelmaati Alsayed , Hosny Hamed Kesba

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Nematology

This research was carried out to study the occurrence of phyto-nematodes associated with medicinal plants. We recorded 7 genera associated with 11 medicinal plant species in plantations of Giza and Fayoum governorates. Susceptibility of 17 medicinal plant species to Meloidogyne incognita and Rotylenchulus reniformis were studied and most plant species were incompatible to both nematode species. The histopathological studies reflected the ranking of medicinal plants from susceptible to immune hosts. The effect of root exudates of seventeen medicinal plant species on hatching and survival of the M. incognita were tested in vitro. Rosemary and marjoram root exudates were the best treatments for inhibiting egg hatch at all concentrations. In vivo experiment, marjoram and rosemary root exudates significantly reduced the nematode criteria in all times of application. Furthermore, immersing juveniles in marjoram exudates for 90 min before inoculation caused approximately 50% reduction in M. incognita infection and fecundity. In micro plots, marjoram was the best in reducing gall formation when intercropped with on sunflower. GC/MS/MS analysis revealed that the most abundant phytochemical compounds in the root exudates of marjoram and rosemary and/or dry leaf extracts of solidago and periwinkle were belonging to classes: phenolics, flavonoids, terpenoids, coumarins, alkaloids and glycosides groups with different concentrations

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