Effects of lactobacillus acidophilus and lactobacillus helveticus on biofilm formation by ESBL-producing uropathogenic escherichia coli / Alaa Mohamed Ibrahim Sayed ; Supervised Magda Ibrahim Mohamed Ayoub , Alaa Mohamed Reda Awad

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Uropathogenic E. coli (UPEC) are important clinical pathogens and are considered as one of the major causes of community-acquired and hospitalacquired urinary tract infection. Unfortunately, the treatment of UPEC infections is becoming more difficult because isolates are increasingly resistant to commonly used antimicrobial agents. The potential ability of UPEC to form biofilm might explain its outstanding antibiotic resistance, survival properties and increased virulence. The aim of the present study was to assess the effect of cell free supernatants (CFS) of Lactobacillus acidophilus La-5 and Lactobacillus helveticus B-734 on the ability of ESBL-producing UPEC isolates to form biofilm in vitro and to eradicate the already formed biofilm of UPEC isolates. A total of 50 isolates of ESBL-producing UPEC, were recovered from different private laboratories, then subcultured on MacConkey{u2019}s medium and identified by conventional microbiological methods. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method and confirmed for ESBL production. The ability of UPEC to produce biofilm was determined using congo red agar method and tissue culture plate method. CFS of L. acidophilus La-5 and L. helveticusB-734 were prepared and used to assess its effect on the ability of ESBL-producing UPEC isolates to form biofilm in vitro and the eradication of the already formed biofilm

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