Thesis (M.Sc.) - Cairo University - Faculty of Medicine- Department of Pediatrics

Background: Neonatal sepsis (NS) is the most common cause of neonatal deaths with a very high mortality despite treatment. With early diagnosis and prompt treatment, mortality and morbidity rates might be reduced, improving the outcome. Signs of sepsis in neonates are often non-specific. Several laboratory parameters (e.g. CBC, CRP and blood cultures) can be helpful for screening of neonates with NS. Cluster of differentiation 14 (CD14) is a glycoprotein expressed on the surface of monocytes, macrophages and neutrophils). CD14 can be divided into soluble CD14 (sCD14) and membrane CD14, sCD14 subtype (sCD14ST) is generated from sCD14 by presepsin. Many studies have found that sCD14-ST could be used as an indicator for early stage sepsis. Many single nucleotide polymorphisms (SNPs) have been identified in the CD14 gene. These polymorphisms mainly include variants in genes coding for proteins involved in the recognition of bacterial pathogens. C-159T polymorphism has been reported to be associated with sepsis by some studies. Objectives: The aim of the study is to evaluate the usefulness of (sCD14-ST) as an early diagnostic biomarker for neonatal sepsis and to detect prevalence of CD14 (-159C/T) gene polymorphism among septic neonates compared to healthy control group. Patients and methods: Our study was carried out on 40 neonates cases with suspected neonatal sepsis at the NICUs of Cairo University Children{u2018}s Hospital,36 neonates cases as control group at gynecology and obstetrics hospital of Cairo University, in the period from January 2019 to October 2019. The results of this study suggested that CD14-159 C/T polymorphism may not significantly influence the risk for sepsis in neonates in Egyptian population

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