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A study of the effects of cigarette smoking on the metabolism of xenobiotics by selected oral microbiota / Hams Atef Mohamed Ahmed Moussa ; Supervised Salwa Ahmed Megahed , Nourtan Fatthy Abdeltawab , Reham Wasfi Sharkawy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hams Atef Mohamed Ahmed Moussa , 2020Description: 147 P. : charts , facsimiles ; 25cmOther title:
  • دراسة تأثير التدخين على عملية ايض الدخائل الحيوية فى بكتيريا مختارة من ميكروبيوم الفم [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology Summary: The composition and metabolic functions of oral microbiota are changed by many factors such as immunity, diet and smoking which in turn can cause local and systemic health problems. One of the health consequences of smoking is alteration and imbalance of the oral microbiota compared to those in non-smokers. Previous studies showed that smoking renders the oral environment rich with microbial genera of phyla as Firmicutes and Actinobacteria, while depleted of others as Proteobacteria, Bacteroidetes and Fusobacteria. Example could be demonstrated by the smoking-dependent altered levels of Veillonella species, phylum Firmicutes, as species of V. dispar showed enrichment while species of V. parvula were depleted. However, it is not known if the depletion of certain bacterial genera and species is due to specific toxins in cigarette smoke, or indirectly due to competition for colonization with smoking enriched bacteria. Moreover, studies have suggested that cigarette smoking may affect the ability of oral microbiota to degrade polycyclic aromatic hydrocarbons (PAHs), however, the exact mechanism remained unknown. Therefore, the aim of the current study was to determine the effect of cigarette smoking on the microbial prevalence and PAHs biodegradation of selected enriched and depleted oral bacteria from oral microbiota compared to that in non-smokers. Samples of oral rinse from smokers and non-smokers were collected (n = 23, 12 smokers and 11 non-smokers) and screened for oral bacterial strains. Viable counts of isolates were compared in smokers vs. non-smokers by growing collected isolates on respective selective media. Cultural, microscopic and molecular identification revealed a total of 69 isolates of Streptococcus mutans, Lactobacillus spp., and Veillonella spp. S mutans, V. tobetsuensis and V. dispar showed higher counts in smokers compared to non-smokers while the Lactobacillus spp. were higher in non-smokers. L. fermentum was prevalent in smokers representing 96.79% of the total isolates
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.M.Sc.2020.Ha.S (Browse shelf(Opens below)) Not for loan 01010110082414000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.M.Sc.2020.Ha.S (Browse shelf(Opens below)) 82414.CD Not for loan 01020110082414000

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

The composition and metabolic functions of oral microbiota are changed by many factors such as immunity, diet and smoking which in turn can cause local and systemic health problems. One of the health consequences of smoking is alteration and imbalance of the oral microbiota compared to those in non-smokers. Previous studies showed that smoking renders the oral environment rich with microbial genera of phyla as Firmicutes and Actinobacteria, while depleted of others as Proteobacteria, Bacteroidetes and Fusobacteria. Example could be demonstrated by the smoking-dependent altered levels of Veillonella species, phylum Firmicutes, as species of V. dispar showed enrichment while species of V. parvula were depleted. However, it is not known if the depletion of certain bacterial genera and species is due to specific toxins in cigarette smoke, or indirectly due to competition for colonization with smoking enriched bacteria. Moreover, studies have suggested that cigarette smoking may affect the ability of oral microbiota to degrade polycyclic aromatic hydrocarbons (PAHs), however, the exact mechanism remained unknown. Therefore, the aim of the current study was to determine the effect of cigarette smoking on the microbial prevalence and PAHs biodegradation of selected enriched and depleted oral bacteria from oral microbiota compared to that in non-smokers. Samples of oral rinse from smokers and non-smokers were collected (n = 23, 12 smokers and 11 non-smokers) and screened for oral bacterial strains. Viable counts of isolates were compared in smokers vs. non-smokers by growing collected isolates on respective selective media. Cultural, microscopic and molecular identification revealed a total of 69 isolates of Streptococcus mutans, Lactobacillus spp., and Veillonella spp. S mutans, V. tobetsuensis and V. dispar showed higher counts in smokers compared to non-smokers while the Lactobacillus spp. were higher in non-smokers. L. fermentum was prevalent in smokers representing 96.79% of the total isolates

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