Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology

The present study aimed to use color and spectral Doppler ultrasound to study both follicular, luteal blood flow vascularization areas in addition to ovarian and uterine arteries Doppler indices in normal spontaneous ovulation and after induction of ovulation using either progesterone releasing intra-vaginal device (PRID - PGF2Ü) or PRID- Modified Co- synch (PRID + GnRH) were evaluated. Nitric oxide and ovarian hormones (progesterone and estrogen) were estimated in blood. Results of the non-induced ovulation showed that both days and phases of the estrous cycle influenced the follicular dynamic, the luteal dynamic, the ovarian and uterine hemodynamics. The highest number of medium and small follicles were found in the early and mid-luteal phase, respectively. However, the follicular phase contained a higher number of large follicles.The present study has demonstrated an important variation in the ovarian hemodynamic in buffaloes between the follicular and luteal phases of the estrous cycle. The follicular phase contained significantly higher follicle area/pixel, antrum area/ pixel, follicle antrum area/pixel, and follicle colored area/pixel than that in luteal one. No significant difference was existed between the blood flow of the dominant follicle and the second largest one.The vascularization of the dominant follicles tended to be higher on the day of estrus. The diameter of CL as well as their blood flow in the present study increased from day 2 of the cycle then gradually elevated to reach the peak at day 14 and decreased to reach a lower value at day 21. The ovarian and uterine blood flow indices (RI, P1, TAMV, S/D, BFV) throughout the estrous cycle did not change in the present study

Issued also as CD