Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Histology

Background and aim of work: Cisplatin is a highly efficient widely used antineoplastic drug in spite of its major serious side effect; acute kidney injury (AKI), so this study was planned to evaluate the therapeutic effect of Mesenchymal stem cells (MSC) derived exosomes on cisplatin induced acute renal injury in adult male albino rats using histological, biochemical, molecular and morphometric studies. Material and Methods: Thirty six male albino rats were divided to 4 groups: Group I (Control group), Group II (Cisplatin-treated group): received a single dose of 6mg/kg body weight cisplatin through an intraperitoneal injection and was sacrificed 1 day from the start of the experiment, Group III (Cisplatin+Exosomes treated group): received a single dose of 6mg/kg body weight cisplatin through an intraperitoneal injection as group II, then after 24 hours, each rat received 200ug exosomes in 0.5ml PBS via the tail vein.Two days later, 3 rats were randomly selected and sacrificed to detect homing of the exosomes, and the rest were sacrificed 5 days from the start of the experiment, Group IV (Spontaneous recovery group): received a single dose of 6mg/kg body weight cisplatin through an intraperitoneal injection as group II and were sacrificed 5 days from the start of experiment. Results: Hematoxylin and Eosin (H&E) stained sections of group II showed extensive glomerular congestion, interstitial extravasation of red blood corpuscles (RBCs), peritubular exudation and cytoplasmic vacuolation in the tubular epithelium. Aggravated tubular epithelial findings with appearance of homogeneous acidophilic masses and widened capsular spaces were detected in group IV. Periodic acid{u2013}Schiff (PAS) stained sections of group II showed loss of brush bordernephrotoxicity

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