Biochemical and molecular biological studies to resistance mechanism in whitefly bemisia tabaci (Gennadius) / Raghda Mohsen Hassan Mohamed ; Supervised Amr Saad Mohamed , Bahia Yehia Reyad , Hala Mohamed Abou Yousef

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Raghda Mohsen Hassan Mohamed

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TextLanguage: English Publication details: Cairo : Raghda Mohsen Hassan Mohamed , 2021Description: 126 P. : charts , facsimiles ; 25cmOther title:

دراسات بيوكيميائية وبيولوجية جزيئية لميكانيكية المقاومة فى حشرة الذبابة البيضاء [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: Because of excessive application of different classes of insecticides for controlling whitefly Bemisia tabaci (Gennadius) [Hemiptera: Aleyrodidae], the development of resistance in laboratory strain of B.tabaci against organophosphours insecticide (primiphos- methyl) were studied for ten generations under laboratory conditions. The resistance ratio (RR) was 22.23 fold in G10 compared with susceptible strain (SS). Also, the development of resistance in seven field populations were collected from Egypt governorates, during season 2018 against different classes of insecticides (pyrethroids, organophosphorus, carbamates and neonicotinoids) were studied. The activity levels of detoxifying enzymes [acetylcholinesterase (AChE), glutathione -S- transferase (GST) and Carboxylesterase (CarE)] were determined in resistance strain and field populations of different governorates, compared to the control. Results showed significant changes in levels of enzymes activity. Applying molecular biological technique to identification the resistance mechanism by studying the role of sodium channel (Sc) and acetylcholinesterase gene (Ace gene) in field populations and resistance strain, the sequences were aligned using Clustal W program, the results showed changes at the level of the nucleotide bases, it was significant, that led to a change in the sequence of the resulting amino acids

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.02.Ph.D.2021.Ra.B (Browse shelf(Opens below))		Not for loan		01010110083013000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.02.Ph.D.2021.Ra.B (Browse shelf(Opens below))	83013.CD	Not for loan		01020110083013000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Because of excessive application of different classes of insecticides for controlling whitefly Bemisia tabaci (Gennadius) [Hemiptera: Aleyrodidae], the development of resistance in laboratory strain of B.tabaci against organophosphours insecticide (primiphos- methyl) were studied for ten generations under laboratory conditions. The resistance ratio (RR) was 22.23 fold in G10 compared with susceptible strain (SS). Also, the development of resistance in seven field populations were collected from Egypt governorates, during season 2018 against different classes of insecticides (pyrethroids, organophosphorus, carbamates and neonicotinoids) were studied. The activity levels of detoxifying enzymes [acetylcholinesterase (AChE), glutathione -S- transferase (GST) and Carboxylesterase (CarE)] were determined in resistance strain and field populations of different governorates, compared to the control. Results showed significant changes in levels of enzymes activity. Applying molecular biological technique to identification the resistance mechanism by studying the role of sodium channel (Sc) and acetylcholinesterase gene (Ace gene) in field populations and resistance strain, the sequences were aligned using Clustal W program, the results showed changes at the level of the nucleotide bases, it was significant, that led to a change in the sequence of the resulting amino acids

Issued also as CD

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