Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Biochemistry

The present study was carried out to evaluate the different biochemical activities of Moringaoleifera and Azadirachtaindica extracts, and their green-chemically synthesized nanoparticles.Chemical composition of dried MoringaandNeemleaves was determined on dry matter which amounted good values of protein, nitrogen free extracts, lipids, ash and minerals. Also, HPLC analysis of Moringaand Neemleaves for the phenols content showed the presence of about 14 compounds in Moringaleavesand 10 compounds in Neemleaves, varying in amount between them.Ag-NPs were prepared in a new developed green-chemically method, and they were characterized by different analytical devices.The antioxidant activity of successive extracts from Moringaand Neemleaves, crude aqueous extracts and synthesized Ag-NPs was determined by DPPH, ABTS and FRAP assays.The antimicrobial activity of successive extracts from Moringaand Neemleaves, crude aqueous extracts and synthesized Ag-NPs was assessed againstthree Gram-positive species and four Gram-negative species of foodborne pathogenic bacteria; Bacillus cereus, Staphylococcus aureus, Staphylococcus sciuri, Salmonella typhi, Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa, and eight phytopathogenic fungal species; Aspergillus flavus, Aspergillus ochraceus, Aspergillus niger, Aspergillus westerdijikia, Aspergillus carbonarius, Aspergillus parasiticus, Penicillium verrucosum and Fusarium proliferatum.Cytotoxicity of Ag-NPs and crude aqueous extracts was determined on macrophage cell line; RAW 264.7, and after lipopolysaccharide treatment, the anti-inflammatory activity was determined by nitric oxide assay.The previous determinations aimed toinvestigate the ability of Moringa and Neem crude aqueous extracts and green-chemically synthesized Ag-NPs to act as promising nutritional,microbiological and cell reinforcement agents

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