Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology

Forkhead box (FOX) proteins are a group of transcriptional factors implicated in different cellular functions such as differentiation, proliferation and senescence. In addition to its role in physiological processes, deregulation of FOX proteins is also involved in the development and progression of tumors raising the possibility that FOX proteins could be used as diagnostic markers and therapeutic targets for cancer. Microtubules are a major component of the eukaryotic cytoskeleton that Microtubules play important roles in virtually every cellular process, such as cell division, cell motility, intracellular organization and trafficking of organelles. Misregulation of such MAPs (Microtubule associated proteins) could interfere with chromosome segregation or cell polarity and potentially contribute to oncogenesis. Aim of the work: To study the diagnostic and prognostic significance of MAP7 and FOXC1 expression in newly diagnosed AML.Methods: Prospective study on 80 AML patients treated at medical oncology departments. FOXC1 and MAP 7genes expression was evaluated by real time RT-PCR using taqman primer probe assay All patients received current standard of care treatment for AML at NCI, Cairo University, Egypt. Results: The median age of the AML patients was {uF0BB}42 years ranging between 18 -65 years. We found that FOXC1was highly expressedin 65/80 (81.3%) of AML patients and high FOXC1 expression was associated with intermediate cytogenetic risk 32(59.3%). The most frequent FAB subtype among high FOXC1 expressers was M2 followed by M4 and M1. High FOXC1 was significantly associated with worse OS (P=0.041), PFS (P=0.024)

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