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Relationship between biofilm formation and production of Extended-Spectrum Ý-lactamases and Metallo-Ý-lactamases among Gram-negative bacilli / Maha Bedair Bassuony Elnaggar ; Supervised Nadia Hafez Ouda , Nermine Samir Rashwan

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Maha Bedair Bassuony Elnaggar , 2021Description: 110 P. : charts , facsmilies ; 25cmOther title:
  • علاقة تكوُن الغشاء الحيوى بإنتاج إنزيمات البيتالاكتاميز ممتدة المدى و إنزيمات الميتالوبيتالاكتاميز فى البكتيريا العصوية سالبة الجرام [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: Gram-negative bacilli are a major cause of healthcare-associated diseases e.g. pneumonia, urinary tract infections and wound infections. However, the treatment of Gram-negative bacilli infections is getting more difficult because of emergence of increasingly resistant isolates to the available antibiotics. The ability of Gram-negative bacilli to form biofilm and produce Ý-lactamases (ESBL and MBL) could be the cause of their virulence and antibiotic resistance. The aim of this study was to detect biofilm, ESBL and MBL production among Gram-negative bacilli, to correlate and assess their antibiotic resistance pattern. Two hundred isolates of Gram-negative bacilli, were recovered from hospitalized patients at Cairo University Hospitals. They were collected from different clinical samples, subcultured on MacConkey{u2019}s agar and identified by the conventional microbiological methods. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. The ability of Gram-negative bacilli to produce biofilm was assessed using microtiter plate method. Gram-negative bacilli isolates were most frequently recovered from urine samples (53%). Biofilm formation was found in 47% of the isolates. Furthermore, the strong biofilm forming category was detected in 2% of the isolates, while the moderate and weak biofilm-forming categories were detected in 13% and 85% respectively. The production of ESBL and MBL among Gram-negative bacilli was 39.5% and 49% respectively. It is concluded that biofilm formation was associated with MBL production among Gram-negative isolates. In addition, ESBL and MBL producers were more resistant to antibiotics than non ESBL and non MBL producers
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.M.Sc.2021.Ma.R (Browse shelf(Opens below)) Not for loan 01010110083456000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.M.Sc.2021.Ma.R (Browse shelf(Opens below)) 83456.CD Not for loan 01020110083456000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Gram-negative bacilli are a major cause of healthcare-associated diseases e.g. pneumonia, urinary tract infections and wound infections. However, the treatment of Gram-negative bacilli infections is getting more difficult because of emergence of increasingly resistant isolates to the available antibiotics. The ability of Gram-negative bacilli to form biofilm and produce Ý-lactamases (ESBL and MBL) could be the cause of their virulence and antibiotic resistance. The aim of this study was to detect biofilm, ESBL and MBL production among Gram-negative bacilli, to correlate and assess their antibiotic resistance pattern. Two hundred isolates of Gram-negative bacilli, were recovered from hospitalized patients at Cairo University Hospitals. They were collected from different clinical samples, subcultured on MacConkey{u2019}s agar and identified by the conventional microbiological methods. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. The ability of Gram-negative bacilli to produce biofilm was assessed using microtiter plate method. Gram-negative bacilli isolates were most frequently recovered from urine samples (53%). Biofilm formation was found in 47% of the isolates. Furthermore, the strong biofilm forming category was detected in 2% of the isolates, while the moderate and weak biofilm-forming categories were detected in 13% and 85% respectively. The production of ESBL and MBL among Gram-negative bacilli was 39.5% and 49% respectively. It is concluded that biofilm formation was associated with MBL production among Gram-negative isolates. In addition, ESBL and MBL producers were more resistant to antibiotics than non ESBL and non MBL producers

Issued also as CD

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