Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry

Background: Mycosis fungoides (MF) is the most frequent cutaneous T-cell lymphoma (CTCL) type, accounting for almost 50% of all CTCLs. The etiology of MF is largely unknown. More recently, a central role for aberrant microRNA (miR) expression has also been suggested. Studies have demonstrated miRNA-specific signatures in different types of CTCL, suggesting that they have a role in the pathogenesis of these disorders. Aim: To detect the expression of miRNA 34a, SIRT-1, p53 & NF{uF06B}B aiming to reveal a possible relation between them and their role in MF pathogenesis. Subjects and Methods: The present study includes 2 main groups: 30 MF patients and 30 unrelated healthy controls. Quantitative expression of serum miR-35a and tissue p53, SIRT-1 and NF{uF06B}B were done to all subjects using real time PCR. Results: The expression of miR 34a and p53 were significantly decreased while the expression of SIRT-1 and NF{uF06B}B were significantly increased in MF patients compared to the control group. There was a statistically significant increase in NF{uF06B}B expression in MF patients having a progressive course compared to those who have a remitting course. Also, there was a significant positive correlation between SIRT-1 and the extent of the lesion.Conclusion: miR 34a plays a role in the pathogenesis of MF as the p53-miR-34a-SIRT1 axis{u2013}dependent manner is the most commonly accepted approach that induces cell apoptosis and senescence in MF

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