Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applicaton in Environmental Metrology Photochemistry Photobiology and Agriculture

Hepatitis C is an infectious liver disease that affects around 180-200 million people worldwide with 2-3 million more people are newly affected annually. The commonly used methods for the detection of HCV include the enzyme-linked immune-sorbent assay (ELISA) and polymerase chain reaction (PCR) techniques. The fluorescence-based detection technique is a central technology in biomedical research and clinical chemistry. The combination of GO nanosheets and fluorescent dyes into a hybrid probe system could have potential applications in environmental monitoring, medical examination and disease diagnostics owing to the advantages of high sensitivity, high selectivity and low-cost of dye/GO-based probes. In this اstudy, a method for the optical spectroscopic detection of hepatitis C virus RNA based on the interaction of GO with dye-labeled HCV RNA and fluorescence change was investigated. A specific probe containing a complementary sequence for mRNA of the HCV 5'UTR gene was designed and utilized with nano graphene oxide as a probe system for the sensing of HCV infection on fluorescence change. A specific DNAzyme probe containing a complementary sequence for mRNA of the HCV 5'UTR gene was utilized with NGO as a probe system for the sensing of HCV infection on fluorescence change was investigated

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