The possible association of some P53-related long non coding RN As and hepatitis C-induced hepatocellular carcinoma in Egyptian patients / Mai Zakaria Mohyeldeen Mahmoud ; Supervised Safinaz Samy Ibrahim , Olfat Gamil Shaker , Hebatullah Samy Mohammed Helmy

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Background: The present study investigated the serum detectability and the diagnostic implications of long noncoding RNAs; nuclear enriched abundant transcript 1 (NEAT1) and taurine upregulatedgene1(TUG1) in viral hepatitis C(HCV)and HCV-induced hepatocellular carcinoma (HCC). Methods: The study included twenty healthy controls, forty non-malignant HCV patients and forty HCV-associated HCC patients.The study assessed liver function tests, the antioxidant status, serumal phafeto protein, p53, NEAT1 and TUG1. Results: Diminished serum expression of NEAT1 and TUG1 was observed in HCV and HCV-induced HCC and was closely associated with deregulated liver function and elevated AFP levels.A model of NEAT1, TUG1 and AFP accurately differentiated between HCC patients and healthy subjects with sensitivity higher than that of AFP alone. Additionally, the diagnostic performance of a model of TUG1, p53 and AFP was superior to that of each marker alone for predicting HCC in HCV patients. Conclusion: Significant alterations in the serum expression of NEAT1 and TUG1 in HCV and HCV-associated HCC patients were recorded. We propose NEAT1 and TUG1 as non-invasive, cost-effective and complementary biomarkers that improve the diagnostic characteristics of AFP

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