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Role of adipose tissue in normal haematopoiesis / Monica Samir Zaky Fahmy ; Supervised Hala Gabr Metwally ; Supervised Hala Gabr Metwally , Asmaa Ahmed Abdelaal , Nevine Fawzy Shafik

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Monica Samir Zaky Fahmy , 2021Description: 113 P. : charts , facsimiles ; 25cmOther title:
  • دور الخلايا الدهنيه فى عمليه تصنيع الدم [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background: Bone Marrow Adipose tissue (BMAT) was previously thought to be just a filler factor, not having any functions. But later on, studies proved that it has a great effect on hematopoiesis, as a part of bone marrow niche. This role was debated by many scientists all over the past ages, between inhibitory and stimulatory effect of BMAT on hematopoiesis. This study was designed to investigate this role.Adipose tissue is considered a promising new source of mesenchymal stem cells. It can be extracted from subcutaneous tissue by liposuction in the plastic cosmetics surgeries, and can be processed to obtain stromal vascular fraction (SVF), which is an aqueous solution containing the stem cells and many other different cells and cytokines. Adipose derived mesenchymal stem cells (AD- MSCs) can be used widely in regenerative medicine, and to facilitate treatment of many diseases. As a substitute for BMAT, we did our study on liposuction fluid, because it is more easily and non-invasive to extract . WE aimed to study the role of adipose tissue in normal hematopoiesis, as a part of the BM niche. Effect of Adipose derived stem cells on HSC was evaluated through co-culture of AD-MSCs (from liposuction) with HSCs (from BM), and co-culture of SVF with HSC. Methodology: Ten donors of bone marrow transplantation, with no hematological disorders, underwent bone marrow aspirate procedure. Ten samples of liposuction fluid, from ten healthy persons were collected. Co-culture of AD-MSCs (from lipoaspirate fluid) was done with HSC (from BM), and expanded for 7 days. Also, direct co-culture of SVF and HSC was done, in 3 dilutions of SVF (1:10, 1:50, 1:100). The effect of AD-MSCs and SVF on HSCs was evaluated by flowcytometry of CD34+, CD38+ and ratio of CD34/CD38. Results: We found that adipose tissue has a great role in hematopoiesis, as it can affect its maturation.The results show significant p-values among (BM only) group and (BM+AD-MSCs) group, regarding CD34 (p-value <0.001), with medians of (1.85 and 11.00) respectively. This shows that AD-MSCs can act as a feeder layer in the in vitro co-cultures and can promote expansion of CD34+ HSC
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2021.Mo.R (Browse shelf(Opens below)) Not for loan 01010110084158000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2021.Mo.R (Browse shelf(Opens below)) 84158.CD Not for loan 01020110084158000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Bone Marrow Adipose tissue (BMAT) was previously thought to be just a filler factor, not having any functions. But later on, studies proved that it has a great effect on hematopoiesis, as a part of bone marrow niche. This role was debated by many scientists all over the past ages, between inhibitory and stimulatory effect of BMAT on hematopoiesis. This study was designed to investigate this role.Adipose tissue is considered a promising new source of mesenchymal stem cells. It can be extracted from subcutaneous tissue by liposuction in the plastic cosmetics surgeries, and can be processed to obtain stromal vascular fraction (SVF), which is an aqueous solution containing the stem cells and many other different cells and cytokines. Adipose derived mesenchymal stem cells (AD- MSCs) can be used widely in regenerative medicine, and to facilitate treatment of many diseases. As a substitute for BMAT, we did our study on liposuction fluid, because it is more easily and non-invasive to extract . WE aimed to study the role of adipose tissue in normal hematopoiesis, as a part of the BM niche. Effect of Adipose derived stem cells on HSC was evaluated through co-culture of AD-MSCs (from liposuction) with HSCs (from BM), and co-culture of SVF with HSC. Methodology: Ten donors of bone marrow transplantation, with no hematological disorders, underwent bone marrow aspirate procedure. Ten samples of liposuction fluid, from ten healthy persons were collected. Co-culture of AD-MSCs (from lipoaspirate fluid) was done with HSC (from BM), and expanded for 7 days. Also, direct co-culture of SVF and HSC was done, in 3 dilutions of SVF (1:10, 1:50, 1:100). The effect of AD-MSCs and SVF on HSCs was evaluated by flowcytometry of CD34+, CD38+ and ratio of CD34/CD38. Results: We found that adipose tissue has a great role in hematopoiesis, as it can affect its maturation.The results show significant p-values among (BM only) group and (BM+AD-MSCs) group, regarding CD34 (p-value <0.001), with medians of (1.85 and 11.00) respectively. This shows that AD-MSCs can act as a feeder layer in the in vitro co-cultures and can promote expansion of CD34+ HSC

Issued also as CD

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