Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Vegetable

This study was carried out in the Tissue Culture Research Laboratory, Horticulture Research Institute, during the period from 2016 to 2020.The study included: (1) Production of microtubers (in vitro tubers), (2) Utilization of microtubers in greenhouse production of minitubers and (3) pre-basic seeds production from minitubers. Two commercial cultivars,{u2018}Diamant{u2019} and {u2018}Spunta{u2019}, were used in the studies. Four tuberization media were used, include MS medium added to it different amount of sucrose, Benzyl adenine (BA), Chloro choline chloride (CCC) and the combination between them. In the three nutrition treatments (MS macro and micronutrients, Hoagland nutrient solution and commercial compound fertilizer) were used in the greenhouse phase. Effects of plant spacing and nitrogen fertilization on pre-basic seeds production were also tested. For microtuberization in vitro the best media was MS supplied with 8% g sucrose. In the greenhouse minitubers production fertilized with Hoagland solution showed the best values regarding minituber weight per plant and average minituber weight.The interaction between 20 cm spacing and 120 kg N/feddan gave the highest values of minitubers number per plot (86.06).The results obtained also revealed that the highest values of minitubers weight per plot (2417.1 g) resulted by interaction between 10 cm spacing and 150 kg N/fed

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