Synthesis and characterization of an electrochemical sensor for highly sensitive determination of the anti-HCV drug daclatasvir dihydrochloride / Hend Abdalla Mohamed Ibrahim Saleh ; Supervised Mahmoud Sabry Rizk , Rasha Mohamed Elnashar

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Analytical Chemistry

This work reports the first electrochemical sensor based on the molecularly imprinted technique for the sensitive and selective determination of the anti{u2012}HCV drug, daclatasvir dihydrochloride (DCT).The electrochemical sensor is fabricated based on the modification of the carbon paste electrode (CPE) with molecularly imprinted methacrylic acid (MAA) as a selective adsorbent of DCT using the ethylene glycol dimethacrylate (EGDMA) as a cross{u2013}linking agent and the reduced graphene oxide (RGO) as a sensitizer.The Hartree{u2012}Fock calculation method has been used for the optimization of template/functional monomer ratios and providing information about the binding of the template to the functional monomer. Cyclic voltammetry, FTIR analysis and scanning electron microscopy (SEM) have been employed for the characterization of the sensor. By the investigation of the optimized analytical parameters, the sensor showed the best performance at pH 2.5, using Britton Robinson (BR) buffer, and a scan rate of 500 mV/s. A good linearity was obtained over the concentration range of 40.6 pg/mL to 812 og/mL with a correlation coefficient of 0.9881. The limits of the detection (LOD) and quantification (LOQ) were found to be 12.2 and 40.2 pg/mL, respectively. The sensor was successfully applied for the determination of DCT in bulk solution, pharmaceutical formulations and human plasma and urine samples. Excellent recovery values ranging from 99.48 to 103.70% were obtained indicating the accuracy and precision of the proposed sensor

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