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Assessment of Ipomoea batatas (L.) Lam. viruses and their potential wild hosts in Egypt / Amira Abdrab Elnaby M. Mazyad ; Supervised Mahasen H. Ismail , Wafaa M. Amer , Amal Aboul Elela A. Farghaly

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Amira Abdrab Elnaby Mohamed Mazyad , 2021Description: 220 P. : charts , facsimiles ; 25cmOther title:
  • تقييم فيروسات البطاطا وعوائلها البرية فى مصر [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology Summary: Globally, sweet potato (Ipomoea batatas (L.) Lam., family Convolvulaceae), is an important vegetable crop, unluckily, the viral infection induces a significant yield loss. The current study aims to identify viruses infecting sweet potato in Egypt and highlights the role of the interfacing weeds as viruses reservoir. The study covered the incidence of the six viruses (SPFMV, SPVG, SPLV, SPMMV, SPCSV and SPLCV) in different governorate in Egypt and focused on the two dominant potyviruses sweet potato feathery mottle virus (SPFMV) and sweet potato virus G (SPVG) in details both of transmission, host range, inclusion bodies, virus morphology, histopathology, serological diagnosis, genome identification using RT-PCR and multiplex RT-PCR. The results revealed that the potyviruses SPFMV and SPVG are the most devastating viruses affecting sweet potato in Egypt. Coinfection with of both viruses was the common feature in both of sweet potato and its interfacing weeds. A wider host range in families Convolvulaceae, Chenopodiaceae and Euphorbiaceae, was detected. The infection symptoms on sweet potato were comparable to that detected in the interfaced weeds. The infection-incidence in perennial Convolvulaceae and Tiliaceae weed-species showed 100% virus incidence. The characteristic amorphous inclusion bodies for the detected viruses and the cytopathological alterations on the host cells were observed under Transmission Electron Microscopy (TEM).The nucleotide sequences of the partial coat protein gene of the detected viruses were compared to the isolate available in GenBank. Next generation sequencing analysis for the complete genome sequence of the two SPFMV and SPVG viruses
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.05.Ph.D.2021.Am.A (Browse shelf(Opens below)) Not for loan 01010110084497000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.05.Ph.D.2021.Am.A (Browse shelf(Opens below)) 84497.CD Not for loan 01020110084497000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

Globally, sweet potato (Ipomoea batatas (L.) Lam., family Convolvulaceae), is an important vegetable crop, unluckily, the viral infection induces a significant yield loss. The current study aims to identify viruses infecting sweet potato in Egypt and highlights the role of the interfacing weeds as viruses reservoir. The study covered the incidence of the six viruses (SPFMV, SPVG, SPLV, SPMMV, SPCSV and SPLCV) in different governorate in Egypt and focused on the two dominant potyviruses sweet potato feathery mottle virus (SPFMV) and sweet potato virus G (SPVG) in details both of transmission, host range, inclusion bodies, virus morphology, histopathology, serological diagnosis, genome identification using RT-PCR and multiplex RT-PCR. The results revealed that the potyviruses SPFMV and SPVG are the most devastating viruses affecting sweet potato in Egypt. Coinfection with of both viruses was the common feature in both of sweet potato and its interfacing weeds. A wider host range in families Convolvulaceae, Chenopodiaceae and Euphorbiaceae, was detected. The infection symptoms on sweet potato were comparable to that detected in the interfaced weeds. The infection-incidence in perennial Convolvulaceae and Tiliaceae weed-species showed 100% virus incidence. The characteristic amorphous inclusion bodies for the detected viruses and the cytopathological alterations on the host cells were observed under Transmission Electron Microscopy (TEM).The nucleotide sequences of the partial coat protein gene of the detected viruses were compared to the isolate available in GenBank. Next generation sequencing analysis for the complete genome sequence of the two SPFMV and SPVG viruses

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