The relationship between hemostatic parameters variation and susceptibility of Bechet{u2019}s disease patients to develop vascular complications / Aya Osama Saad Hassan ; Supervised Hanan Nour Ahmed Raslan , Sherif Mohamed Yousry , Maha Hamdi Elsissy

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Bechet{u2019}s disease (BD) is a multisystem vascular inflammatory disorder with frequent exacerbations and remissions. The pathogenesis of thrombosis in BD is not clearly known. Vasculitis is the predominant histopathological feature of the disease. The management of vascular thrombosis in BD patients is based on immunosuppressive drugs in addition to anticoagulant and anti-platelet therapy in cases associated with thrombophilic insults. Aim: to investigate the role of hypercoagulable state and/or the hemostatic alteration in BD patients and their susceptibility to develop vascular thrombotic complications Methods: We included 45 patients with BD, further divided into 2 groups based on the presence of vascular events. they underwent molecular detection of thrombophilia gene mutation by polymerase chain reaction (PCR) and reverse-hybridization to allele-specific oligonucleotide probes immobilized on test strip. Natural anticoagulants levels as protein C, protein S & antithrombin and MPV were also measured to assess the association between activated coagulation system and increased susceptibility of Bechet{u2019}s Disease patients to develop vascular complication. Results: a significant association between HPA-1 polymorphism and vascular thrombosis either arterial or venous in BD patients was detected. No statistically significant association was found between vascular thrombosis in BD and the other studied thrombophilic gene mutation. There was an insignificant association between thrombosis incidence in BD patients with levels of natural anticoagulants (protein C, protein S, antithrombinIII) and MPV level

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