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Effect of taurine-loaded nanoparticles on acetic acid-induced colitis in rats : Immunological study / Osama Ahmed Galal Mohamed ; Supervised Azza Mohamed Elamir , Alyaa Ahmed Farid

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Osama Ahmed Galal Mohamed , 2021Description: 137 P. : charts , facsimiles ; 25cmOther title:
  • تأثير التاورين المحمل على جزيئات النانو على إلتهاب القولون المستحث بحامض الخليك فى الجرذان : دراسة مناعية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: Owing to the poor outcomes and adverse side effects of existing ulcerative colitis drugs, developing an alternative nano-based treatment approach is highly warranted. Therefore, this proposal was designed to characterize the in vitro and in vivo properties of taurine, taurine-loaded chitosan pectin nanoparticles (Tau-CS-PT-NPs), and chitosan pectin nanoparticles (CS-PT-NPs) in the treatment of colitis induced by acetic acid in rats. For this purpose, CS-PT-NPs and Tau-CS-PT-NPs were prepared using the ionic gelation method and characterized by transmission electron microscopy (TEM), polydispersity index (PDI), zeta potential, Fourier transform infrared (FTIR) spectroscopy, encapsulation efficiency (EE), and drug release profile. Following colitis induction, rats were treated orally with free taurine, Tau-CS-PT-NPs, and CS-PT-NPs once per day for six days. Data showed that the size of Tau-CS-PT-NPs significantly increased compared to CS-PT-NPs. PDI and zeta potential values were 0.419, +48.4mV for CS-PT-Tau-NPs and 0.523, +43.5mV for CS-PT-NPs. EE was about 69.09±1.58%, and 60% of taurine was released in 4 h in simulated colon content. Acetic acid-induced colitis in untreated rats caused necrosis of colon tissues and a significant increase in interleukin-1beta, tumor necrosis factor-alpha, myeloperoxidase, and malondialdehyde levels associated with a remarkable decrease in reduced glutathione level in colon tissue in comparison to the control group, while, taurine, Tau-CS-PT-NPs, and CS-PT-NPs treatment partly reversed these effects. Our present study demonstrated that the administration of free taurine, CS-PT-NPs, and Tau-CS-PT-NPs exerted beneficial effects in acetic acid-induced colitis by their anti-inflammatory and antioxidant activities.Overall, taurine-loaded chitosan pectin nanoparticles emerge as a promising therapeutic option for colitis
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2021.Os.E (Browse shelf(Opens below)) Not for loan 01010110084970000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2021.Os.E (Browse shelf(Opens below)) 84970.CD Not for loan 01020110084970000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Owing to the poor outcomes and adverse side effects of existing ulcerative colitis drugs, developing an alternative nano-based treatment approach is highly warranted. Therefore, this proposal was designed to characterize the in vitro and in vivo properties of taurine, taurine-loaded chitosan pectin nanoparticles (Tau-CS-PT-NPs), and chitosan pectin nanoparticles (CS-PT-NPs) in the treatment of colitis induced by acetic acid in rats. For this purpose, CS-PT-NPs and Tau-CS-PT-NPs were prepared using the ionic gelation method and characterized by transmission electron microscopy (TEM), polydispersity index (PDI), zeta potential, Fourier transform infrared (FTIR) spectroscopy, encapsulation efficiency (EE), and drug release profile. Following colitis induction, rats were treated orally with free taurine, Tau-CS-PT-NPs, and CS-PT-NPs once per day for six days. Data showed that the size of Tau-CS-PT-NPs significantly increased compared to CS-PT-NPs. PDI and zeta potential values were 0.419, +48.4mV for CS-PT-Tau-NPs and 0.523, +43.5mV for CS-PT-NPs. EE was about 69.09±1.58%, and 60% of taurine was released in 4 h in simulated colon content. Acetic acid-induced colitis in untreated rats caused necrosis of colon tissues and a significant increase in interleukin-1beta, tumor necrosis factor-alpha, myeloperoxidase, and malondialdehyde levels associated with a remarkable decrease in reduced glutathione level in colon tissue in comparison to the control group, while, taurine, Tau-CS-PT-NPs, and CS-PT-NPs treatment partly reversed these effects. Our present study demonstrated that the administration of free taurine, CS-PT-NPs, and Tau-CS-PT-NPs exerted beneficial effects in acetic acid-induced colitis by their anti-inflammatory and antioxidant activities.Overall, taurine-loaded chitosan pectin nanoparticles emerge as a promising therapeutic option for colitis

Issued also as CD

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