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Study of microrna 92a expression in patients with colorectal cancer / Ahmed Kamal Zaki Elywa ; Supervised Samia Yehia Akel , Amal Fawzy Said , Reham Aly Elshimy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ahmed Kamal Zaki Elywa , 2021Description: 108 P. : charts , facsimiles ; 25cmOther title:
  • دراسة التعبير الجينى للحمض النووى الريبوزى متناهى الصغر(92أ) فى حالات سرطان القولون والمستقيم [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology Summary: Background: Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies and leading causes of cancer related deaths all over the world. MicroRNAs can regulate more than 60% of human genes, including oncogenes and tumor suppressor genes. Therefore, they can promote tumor development and affect risk of malignancy. The abnormal high expression of the oncogenic microRNA, miR-92a in colorectal cancer promotes tumor proliferation, metastasis, and invasion via targeting and inhibiting different tumor apoptosis proteins including the anti-apoptotic molecule BCL-2-interacting mediator of cell death (Bim).Aim of the work: This study aimed to assess the role of serum miR-92a as a non-invasive tumor marker in clolorectal cancer (CRC) patients, outline the correlation between miR-92a and the pro-apoptotic protein BCL-2-interacting mediator of cell death (Bim) as one of its target proteins, and to determine their associative relations with other clinicopathological parameters in CRC and adenoma patients.Materials and Methods: A total of 54 newly diagnosed CRC patients,15 colonic adenoma patients, and 15 age-matched apparently healthy volunteering controls were recruited in this study. MiR-92a levels were assayed by TaqMan- Real time RT-PCR method and Bim levels were measured by ELISA. Results: Significant higher expression of serum miR-92a levels were observed in CRC patients as compared to the adenoma and control groups (p <0.001 each) and lower serum Bim levels in CRC patients as compared to the adenoma and control groups (p=0.001, p <0.001 respectively).The ROC curve analysis showed excellent AUC for plasma miR-92a in discriminating CRC from control (AUC=0.994), and adenoma (AUC=0.993) groups with the highest diagnostic performance in discriminating CRC from control (at cut off 1.43, sensitivity 98.1%, specificity 93.9%), and adenoma (at cut off 1.78, sensitivity 92.6%, specificity 93.3%)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.19.03.Ph.D.2021.Ah.S (Browse shelf(Opens below)) Not for loan 01010110085196000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.19.03.Ph.D.2021.Ah.S (Browse shelf(Opens below)) 85196.CD Not for loan 01020110085196000

Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology

Background: Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies and leading causes of cancer related deaths all over the world. MicroRNAs can regulate more than 60% of human genes, including oncogenes and tumor suppressor genes. Therefore, they can promote tumor development and affect risk of malignancy. The abnormal high expression of the oncogenic microRNA, miR-92a in colorectal cancer promotes tumor proliferation, metastasis, and invasion via targeting and inhibiting different tumor apoptosis proteins including the anti-apoptotic molecule BCL-2-interacting mediator of cell death (Bim).Aim of the work: This study aimed to assess the role of serum miR-92a as a non-invasive tumor marker in clolorectal cancer (CRC) patients, outline the correlation between miR-92a and the pro-apoptotic protein BCL-2-interacting mediator of cell death (Bim) as one of its target proteins, and to determine their associative relations with other clinicopathological parameters in CRC and adenoma patients.Materials and Methods: A total of 54 newly diagnosed CRC patients,15 colonic adenoma patients, and 15 age-matched apparently healthy volunteering controls were recruited in this study. MiR-92a levels were assayed by TaqMan- Real time RT-PCR method and Bim levels were measured by ELISA. Results: Significant higher expression of serum miR-92a levels were observed in CRC patients as compared to the adenoma and control groups (p <0.001 each) and lower serum Bim levels in CRC patients as compared to the adenoma and control groups (p=0.001, p <0.001 respectively).The ROC curve analysis showed excellent AUC for plasma miR-92a in discriminating CRC from control (AUC=0.994), and adenoma (AUC=0.993) groups with the highest diagnostic performance in discriminating CRC from control (at cut off 1.43, sensitivity 98.1%, specificity 93.9%), and adenoma (at cut off 1.78, sensitivity 92.6%, specificity 93.3%)

Issued also as CD

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