Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Medical Biological

Objective: Studying the effect Low Level Laser Therapy on the mice epithelial endometrial organoids regarding cell proliferation, endometrial regeneration, and endometrial receptivity. Studying the role of epithelial progesterone receptors (PGR) on implantation and endometrial receptivity using genetically modified mice model with only epithelial deletion of PGR (ePGR- KO).Study design: the in vitro part was to generate mice epithelial organoid model and testing LLLT using He:NE 632.8 nm device and testing cell proliferation and organoid function markers then culturing organoids with DUM to test the regeneration ability of organoids to endometrial tissue and the effect of LLLT on regeneration.The genetically modified animal model ePGR -KO, to test ePGR effect on endometrial receptivity and implantation. Both stages are testing the morphological and hormonal aspects of endometrial receptivity Results: LLLT proved to have a proliferative effect on the epithelial mouse organoids confirmed by Ki67 and PCNA IHC and ePGR are a mandatory mediator that regulate both endometrial epithelial and stromal function for successful implantation. Conclusion: LLLT and organoids are a promising modality to treat Asherman syndrome correcting the morphology of the endometrium for receptive uterus. Also, our results address a critical role of epithelial PGR for uterine receptivity and embryo attachment in early pregnancy

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