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Impact of diabetes type II on the anti-inflammatory properties of human derived adipose mesenchymal stem cells / Nourhan Mohamed Diaa Eldien Abushahba ; Supervised Amel Ibrahim Othman , Khalda Sayed Amr , Osama Mahmoud Azmy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Nourhan Mohamed Diaa Eldien Abushahba , 2021Description: 119 P . : charts ; 25cmOther title:
  • تأثير النوع الثانى لداء السكرى على الخصائص المضادة للالتهاب للخلايا الجذعية الوسيطة المستمدة من النسيج الدهنى فى الإنسان [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology Summary: Type 2 diabetes mellitus (T2DM) is a serious chronic metabolic disorder. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) possess immunomodulatory/anti-infammatory potential rendering them useful for treating inflammation-related disorders such as T2DM. However, the microenvironment of T2DM may affect their therapeutic potential. This study examined the impact of the diabetic milieu on the anti-inflammatory potential of AT-MSCs. Our data revealed a lower expression of CD200 and CD276 on diabetic AT-MSCs (dAT-MSCs) relative to non-diabetic AT-MSCs (ndAT-MSCs) as demonstrated by flow cytomety. qPCR showed upregulation of IL-1Ý associated with downregulation of IL-1RN mRNA expression in dAT-MSCs vs. ndAT-MSCs. ELISA analysis uncovered elevated levels of secreted IL-1Ý, TNF, and visfatin/NAMPT in dAT-MSCs, whereas IL-1RA and IDO levels were reduced. IFN-Þ priming induced an elevation in CD274 expression, IDO1 and IL-1RN overexpression and IL-1Ý downregulation in both groups. ELISA results were also evident in the secretome of dAT-MSCs upon IFN-Þ priming. In conclusion, T2DM milieu alters the immunomodulatory characteristics of AT-MSCs with a shift towards a proinflammatory phenotype which may restrain their autologous therapeutic use. However, IFN-Þ priming could be a useful strategy for enhancing their anti-inflammatory potential
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2021.No.I (Browse shelf(Opens below)) Not for loan 01010110085372000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2021.No.I (Browse shelf(Opens below)) 85372.CD Not for loan 01020110085372000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology

Type 2 diabetes mellitus (T2DM) is a serious chronic metabolic disorder. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) possess immunomodulatory/anti-infammatory potential rendering them useful for treating inflammation-related disorders such as T2DM. However, the microenvironment of T2DM may affect their therapeutic potential. This study examined the impact of the diabetic milieu on the anti-inflammatory potential of AT-MSCs. Our data revealed a lower expression of CD200 and CD276 on diabetic AT-MSCs (dAT-MSCs) relative to non-diabetic AT-MSCs (ndAT-MSCs) as demonstrated by flow cytomety. qPCR showed upregulation of IL-1Ý associated with downregulation of IL-1RN mRNA expression in dAT-MSCs vs. ndAT-MSCs. ELISA analysis uncovered elevated levels of secreted IL-1Ý, TNF, and visfatin/NAMPT in dAT-MSCs, whereas IL-1RA and IDO levels were reduced. IFN-Þ priming induced an elevation in CD274 expression, IDO1 and IL-1RN overexpression and IL-1Ý downregulation in both groups. ELISA results were also evident in the secretome of dAT-MSCs upon IFN-Þ priming. In conclusion, T2DM milieu alters the immunomodulatory characteristics of AT-MSCs with a shift towards a proinflammatory phenotype which may restrain their autologous therapeutic use. However, IFN-Þ priming could be a useful strategy for enhancing their anti-inflammatory potential

Issued also as CD

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