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Preparation And Evaluation Of Mycoplasma And Salmonella Vaccines For Chickens / Muhammed Saeed Mostafa Elnaggar ; Supervised Sherif Abdelmonaem Omar Marouf , Heba Mohammed Mohammed Salem , Hazem Mohammed Ibrahim

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Muhammed Saeed Mostafa Elnaggar , 2022Description: 98 P . : charts , photographs ; 25cmOther title:
  • تحضير وتقييم لقاحات لميكروبى الميكوبلازما والسالمونيلا في الدجاج [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of veterinary medicine - Department of Microbiology Summary: In the chicken industry Mycoplasma and Salmonella are vertically transmitted diseases that cause significant economic losses, so this study aimed to prepare locally an inactivated bivalent Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) vaccine and pentavalent Salmonella Typhimurium (S.T.), Salmonella Entriditis (S.E.), Salmonella Kentucky (S.K.), (MG) and (MS) vaccine also, trivalent S.T., S.E. and S.K. commercially available vaccine was evaluated. The prepared bivalent and pentavalent vaccines were adjuvanted with Montanide ISA70 then tested for safety, sterility, and potency. Both vaccines efficacy was evaluated in 160 SPF one day old chicks. Chicks were vaccinated with 1st dose at a week of age then boostered with the 2nd dose after 3 weeks. Three weeks post vaccination with 2nd dose chicks were challenged, the birds were kept under observation for 10 weeks. During the experiment clinical symptoms, daily mortalities, postmortem examination, and weights were recorded as well as, serum, cloacal swabs and nasal swabs were collected. Mycoplasma and Salmonella strains humoral immune response was assessed using serum samples taken weekly after first immunization until the third week after challenge measured by the enzyme-linked immunosorbent assay (ELISA) test and results showed that vaccines gave a high protective antibody titer. We concluded that locally manufactured inactivated bivalent Mycoplasma vaccine gave 85% protection, trivalent Salmonella commercially available vaccine gave 80% protection and pentavalent vaccine gave 100% protection. Based on our results, we recommend using of a locally prepared inactivated pentavalent vaccine to control of mycoplasmosis and salmonellosis in layers and breeders chicken farms in Egypt.
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2022.Mu.P (Browse shelf(Opens below)) Not for loan 01010110085672000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2022.Mu.P (Browse shelf(Opens below)) 85672.CD Not for loan 01020110085672000

Thesis (M.Sc.) - Cairo University - Faculty of veterinary medicine - Department of Microbiology

In the chicken industry Mycoplasma and Salmonella are vertically transmitted diseases that cause significant economic losses, so this study aimed to prepare locally an inactivated bivalent Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) vaccine and pentavalent Salmonella Typhimurium (S.T.), Salmonella Entriditis (S.E.), Salmonella Kentucky (S.K.), (MG) and (MS) vaccine also, trivalent S.T., S.E. and S.K. commercially available vaccine was evaluated. The prepared bivalent and pentavalent vaccines were adjuvanted with Montanide ISA70 then tested for safety, sterility, and potency. Both vaccines efficacy was evaluated in 160 SPF one day old chicks. Chicks were vaccinated with 1st dose at a week of age then boostered with the 2nd dose after 3 weeks. Three weeks post vaccination with 2nd dose chicks were challenged, the birds were kept under observation for 10 weeks. During the experiment clinical symptoms, daily mortalities, postmortem examination, and weights were recorded as well as, serum, cloacal swabs and nasal swabs were collected. Mycoplasma and Salmonella strains humoral immune response was assessed using serum samples taken weekly after first immunization until the third week after challenge measured by the enzyme-linked immunosorbent assay (ELISA) test and results showed that vaccines gave a high protective antibody titer. We concluded that locally manufactured inactivated bivalent Mycoplasma vaccine gave 85% protection, trivalent Salmonella commercially available vaccine gave 80% protection and pentavalent vaccine gave 100% protection. Based on our results, we recommend using of a locally prepared inactivated pentavalent vaccine to control of mycoplasmosis and salmonellosis in layers and breeders chicken farms in Egypt.

Issued also as CD

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