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Trials for Vaccine Preparation Against Tuberculosis / Mohamed Abd El rahman Mohamed Osman ; Supervised Salah ElDien Abd El Karim Selim , Magdy Ahmed El Sayed Ghoneim , Essam Amin Nasr

By: Contributor(s): Language: Eng Publication details: Cairo : Mohamed Abdelrahman Mohamed Osman , 2006Description: 197P : ill ; 30cmOther title:
  • محاولات تحضير لقاح ضد مرض السل [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (PH.D.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology Summary: The current study aimed for the cloning and expression of ESAT - 6 protein antigen from Mbovis and its use alone or plus adjuvant as a vaccine against bovine tuberculosisPrimarily , Mbovis genomic DNA was extracted using triazol purified , concentrated and then esat - 6 gene was amplified by PCR and assessed by agarose gel electrophoesisesat - 6 gene was ligated to an expression vector , PQEAfter transformation by electroporation of the expression host Ecoli with the recombinant PQE plasmid , the expression was induced using 10 mM of IPTG , two bands were seen in the SDS - PAGE analysis , the 18 kDa which represented the dimmer forms of their ESAT - 6 antigenThe his - tagged r - ESAT - 6 antigen was then purified by metal affinity chromatography using Ni - NTA agarose
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Item type Current library Home library Call number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2006.Mo.T. (Browse shelf(Opens below)) Not for loan 01010110045050000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2006.Mo.T. (Browse shelf(Opens below)) Not for loan 01020110045050000

Thesis (PH.D.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology

The current study aimed for the cloning and expression of ESAT - 6 protein antigen from Mbovis and its use alone or plus adjuvant as a vaccine against bovine tuberculosisPrimarily , Mbovis genomic DNA was extracted using triazol purified , concentrated and then esat - 6 gene was amplified by PCR and assessed by agarose gel electrophoesisesat - 6 gene was ligated to an expression vector , PQEAfter transformation by electroporation of the expression host Ecoli with the recombinant PQE plasmid , the expression was induced using 10 mM of IPTG , two bands were seen in the SDS - PAGE analysis , the 18 kDa which represented the dimmer forms of their ESAT - 6 antigenThe his - tagged r - ESAT - 6 antigen was then purified by metal affinity chromatography using Ni - NTA agarose

Issued also as CD

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