TY - BOOK AU - Brown,T.A. TI - Gene cloning and DNA analysis: an introduction SN - 1405111216 (pbk. : alk. paper) AV - QH442.2 .B76 2006 PY - 2006/// CY - Oxford, Malden, MA PB - Blackwell Pub. KW - DNA KW - Analysis KW - Molecular cloning KW - Nucleotide sequence KW - Cloning, Molecular KW - DNA, Recombinant KW - analysis KW - Sequence Analysis, DNA N1 - Includes bibliographical references and index; PART 1: THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS: Why gene cloning and DNA are important -- Vectors for gene cloning: plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for E. coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- PART 2: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN RESEARCH: Studying gene location and structure -- Studying gene expression and function -- Studying genomes -- PART 3: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN BIOTECHNOLOGY: Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology -- Glossary ER -