Bassant Alaa Eldin Gomaa, <br/><br/>
Control of multi-drug resistant escherichia coli infecting the urinary tract in children /  
 مقاومه بكتريا الاشرشيا القولونية المقاومة للدواء والتي تصيب المجرى البولي للألطفال 
Presented by Bassant Alaa Eldin Gomaa ; supervisors Prof. Amira Mohamed AbuTaleb, Dr. Sherif Mohamed Elnagdy. 
 - 116 pages :   illustrations ;   25 cm. +   CD. 
<br/><br/>Thesis (M.Sc.)-Cairo University, 2022.   
<br/><br/>Bibliography: pages 90-115.  
<br/><br/> The UTI is one of the most common infections in<br/>children. Out of 850 urine samples, were collected from children suspected to have<br/>urinary tract infection (UTI), 400 samples showed positive cultures. Only 287 (71.75<br/>%) cases out of 400 were diagnosed as the UTI. The UTI was more common among<br/>females 173/287 (60.3 %) than males 114/287 (39.7 %). Children at the age between<br/>3–6 years old were the most susceptible to infection compared to other ages.<br/>Escherichia coli was the main causative pathogen 115/287 (40.06 %) followed by<br/>Klebsiella 50/287 (17.4 %). All isolates of E. coli were resistant to amoxicillin and<br/>ticarcillin. On the other hand, amikacin, meropenem, nitrofurantoin and imipenem<br/>were the most efficient antibiotics and succeeded in controlling high number of E. coli<br/>isolates (88.69 % - 96.52 %). Non ESBL non AmpC producing E. coli isolates were<br/>more susceptible to antibiotics and essential oils than ESBL and AmpC producing E.<br/>coli isolates. Cinnamon oil was the most effective oil against the different strains of E.<br/>coli (ESBL, AmpC and non ESBL non AmpC), followed by thyme, clove, rosemary,<br/>lemon grasses and peppermint. On the other hand, oils of castor, lavender, anise, and<br/>cumin were ineffective against the three strains of E. coli. It was found that MICs<br/>were decreased in cinnamon oil/antibiotic combinations compared to the oil or<br/>antibiotic separately. Fractional inhibitory concentrations indices (FICIs) were<br/>calculated to determine the interaction between cinnamon oil and the selected<br/>antibiotics against the multidrug resistant isolates of E. coli. The synergistic effect<br/>was observed only in cinnamon oil/gentamycin combination in all strains of E. coli<br/>(P-value <0.001). The cnf1 virulence gene was detected by PCR technique. However,<br/>the resistance of the ESBL and AmpC β-lactamase producing E. coli to antibiotics<br/>was statically non-significant in relation to the presence of the cnf1 gene. The lowest<br/>distribution of cnf1 gene was found among non-ESBL non-AmpC producing E. coli<br/>to the antibiotics was significantly related to the presence of the cnf1 gene.  يعد التهاب المسالك البولية أحد أكثر أنواع العدوى شيوعًا في<br/>أطفال. تم جمع 850 عينة بول من أطفال يشتبه في إصابتهم بالمرض<br/>التهاب المسالك البولية (UTI)، أظهرت 400 عينة إيجابية الزرع. فقط 287 (71.75<br/>%) من أصل 400 حالة تم تشخيصها على أنها التهاب المسالك البولية. وكان التهاب المسالك البولية أكثر شيوعا بين<br/>الإناث 173/287 (60.3%) مقابل الذكور 114/287 (39.7%). الأطفال في سن ما بين<br/>وكان الأطفال من 3 إلى 6 سنوات هم الأكثر عرضة للإصابة مقارنة بالأعمار الأخرى.<br/>كانت الإشريكية القولونية هي المسبب الرئيسي للمرض 115/287 (40.06%) يليها<br/>كليبسيلا 50/287 (17.4%). كانت جميع عزلات بكتريا E. coli مقاومة للأموكسيسيلين و<br/>تيكارسيلين. ومن ناحية أخرى، أميكاسين، ميروبينيم، نيتروفورانتوين وإيميبينيم<br/>كانت المضادات الحيوية الأكثر فعالية ونجحت في السيطرة على عدد كبير من بكتيريا الإشريكية القولونية 
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<br/><br/><br/>Text in English and abstract in Arabic & English. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Escherichia coli infections.
<br/><br/><label>Subjects--Index Terms: </label>cinnamon oil  non ESBL non AmpC producing E. coli  synergistic effect  necrotoxin gene 
<br/><br/><label>Dewey Class. No.: </label>616.01442