Reem Maged Ahmed Sheta, <br/><br/>
Estimation of the safety and antitumor activity of silver doped hydroxyapatite nanoparticles in mice /  
 ﺗﻘدﯾر اﻟﺳﻼﻣﺔ واﻟﻧﺷﺎط اﻟﻣﺿﺎد ﻟﻠورم ﻣن ﺟزﯾﺋﺎت ھﯾدروﻛﺳﯾﺑﺎﺗﯾت اﻟﻧﺎﻧوﯾﺔ اﻟﻣطﻌﻣﺔ ﺑﺎﻟﻔﺿﺔ ﻓﻲ اﻟﻔﺋران 
by Reem Maged Ahmed Sheta ; supervised by Ass. Prof. Hanan Ramadan Hamad, Prof. Gehan Safwat ElHusseiny. 
 - 135 pages :   illustrations ;   25 cm. +   CD. 
<br/><br/>Thesis (M.Sc.)-Cairo University, 2023.  
<br/><br/>Bibliography: pages 119-131. 
<br/><br/> Currently, the main therapies for treating cancer are associated with a variety of side effects.<br/>Accordingly, researchers are focusing on the use of nanotherapy to develop a highly novel, less toxic<br/>and extremely efficient therapeutic agents for cancer treatment. Silver doped Hydroxyapatite<br/>nanoparticles (Ag:HAp-NPs) have shown effective at treating both infections and cancerous tumors<br/>while being non- cytotoxic to normal healthy cells in vitro. Therefore, this study was undertaken to<br/>estimate the safety and anti-tumor activity of silver doped hydroxyapatite nanoparticles in mice in<br/>vivo. Regarding the antitumor activity, concurrent administration of Ag:HAp-NPs caused a<br/>significant decrease in tumor volume and weight. Additionally, it showed a high elevation of<br/>intracellular ROS generation as well as MDA levels within tumor tissues and lead to the induction<br/>of DNA breaks. The high production of intracellular ROS generation, and the oxidative stress<br/>induction by Ag:HAp-NPs within tumor tissues confirmed a decrease in GSH and GPx levels.<br/>Significant elevation of P53 and HO-1 gene and decrease in Hsp70 and Kras was observed as well.<br/>Additionally, the percentage of necrotic tissue in tumor- induced groups and % of degenerated tumor<br/>cells after treatment with Ag:HAp-NPs were significantly elevated. Regarding the safety of Ag:HAp-<br/>NPs, it was shown to be safe and non-genotoxic. This was confirmed by the non-significant changes<br/>in TL and TM in the bone marrow and liver tissues. It was further confirmed by the observed non-<br/>significant changes in the MDA and TAC levels as well as the SOD activity in the liver among the<br/>safety groups, except for high dosage level. Moreover, histological examinations demonstrated that<br/>Silver doped Hydroxyapatite nanoparticles have mild toxicity on kidney and liver tissues with<br/>minimal inflammatory reactions associated with the presence of Ag:HAp-NPs in contrast with<br/>Cisplatin. In conclusion, silver doped hydroxyapatite nanoparticles have a potential anticancer<br/>activity against induced solid tumors in mice and demonstrated a higher level of safety for the liver,<br/>kidney and bone marrow tissues, making Ag:HAp-NPs a promising anti-tumor agent.  ﺣﺎﻟًﯿﺎ،ﺗﺮﺗﺒﻂ اﻟﻌﻼﺟﺎت اﻟﺮﺋﯿﺴﯿﺔ ﻟﻌﻼج اﻟﺴﺮطﺎن ﺑﻤﺠﻤﻮﻋﺔ ﻣﺘﻨﻮﻋﺔﻣﻦاﻵﺛﺎر اﻟﺠﺎﻧﺒﯿﺔ.وﻓًﻘﺎ ﻟﺬﻟﻚ،ﯾﺮﻛﺰا ﻟﺒﺎﺣﺜﻮن ﻋﻠﻰ<br/>اﺳﺘﺨﺪام اﻟﻌﻼج ﺑﺎﻟﻨﺎﻧﻮ ﻟﺘﻄﻮﯾﺮ ﻋﻮاﻣﻞ ﻋﻼﺟﯿﺔ ﺟﺪﯾﺪة ﻟﻠﻐﺎﯾﺔ ؤاﻗﻞ ﺳﻤﯿﺔ وﻓﻌﺎﻟﺔ ﻟﻠﻐﺎﯾﺔ ﻟﻌﻼج اﻟﺴﺮطﺎن.ٔاظﮭﺮت ﺟﺰﯾﺌﺎت<br/>ھﯿﺪروﻛﺴﯿﺒﺎﺗﯿﺖ اﻟﻨﺎﻧﻮﯾﺔ اﻟﻤﻄﻌﻤﺔ ﺑﺎﻟﻔﻀﺔ)Ag: HAp-NPs( ﻓﻌﺎﻟﯿﺘﮭﺎ ﻓﻲ ﻋﻼج ﻛﻞ ﻣﻦ اﻻﻟﺘﮭﺎﺑﺎت واﻷورام اﻟﺴﺮطﺎﻧﯿﺔ<br/>ﺑﯿﻨﻤﺎ ﺗﻜﻮن ﻏﯿﺮﺳﺎﻣﺔ ﻟﻠﺨﻼﯾﺎ اﻟﺴﻠﯿﻤﺔ ﻣﻌﻤﻠﯿﺎ in vitro .ﻟﺬﻟﻚ،ٔ اﺟﺮﯾﺖ ھﺬه اﻟﺪراﺳﺔ ﻟﺘﻘﺪﯾﺮ اﻟﺴﻼﻣﺔ واﻟﻨﺸﺎط اﻟﻤﻀﺎد ﻟﻠﻮرم<br/>ﻣﻦ ﻟﺠﺴﯿﻤﺎت ھﯿﺪروﻛﺴﯿﺒﺎﺗﯿﺖ اﻟﻨﺎﻧﻮﯾﺔ اﻟﻤﻄﻌﻤﺔ ﺑﺎﻟﻔﻀﺔ ﻓﻲ اﻟﻔﺌﺮان ﻓﻲ اﻟﺠﺴﻢ اﻟﺤﻲ in vivo .ﻓﯿﻤﺎ ﯾﺘﻌﻠﻖ ﺑﺎﻟﻨﺸﺎط اﻟﻤﻀﺎد<br/>ﻟﻠﻮرم، ﺗﺴﺒﺐ اﻹ ﻋﻄﺎء اﻟﻤﺘﺰاﻣﻦ ﻟـ Ag: HAp-NPs ﻓﻲ اﻧﺨﻔﺎض ﻛﺒﯿﺮ ﻓﻲ ﺣﺠﻢ ووزن اﻟﻮرم. ﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰ ذﻟﻚ اظﮭﺮ<br/>ارﺗﻔﺎﻋﺎ ﻛﺒیرا ﻓﻲﺗﻮﻟﯿﺪ ROSداﺧﻞ اﻟﺨﻼﯾﺎ وﻛﺬﻟﻚ ﻣﺴﺘﻮﯾﺎتMDA داﺧل أﻧﺴﺠﺔ اﻟﻮرم وﯾٔﻮديٕ اﻟﻰ ﺗﺤﺮﯾﺾ ﻓﻮاﺻﻞ<br/>اﻟﺤﻤﺾاﻟﻨﻮوي.ٔ اﻛﺪ اﻹﻧﺘﺎج اﻟﻌﺎﻟﻲﻟﺘﻮﻟﯿﺪ ROS داﺧﻞ اﻟﺨﻼﯾﺎ، وﺗﺤﺮﯾﺾ اﻹﺟﮭﺎد اﻟﺘﺄﻛﺴﺪي ﺑﻮاﺳﻄﺔ Ag: HAp-NPs<br/>داﺧﻞٔ اﻧﺴﺠﺔاﻟﻮرم اﻧﺨﻔﺎﺿا ﻓﻲ ﻣﺴﺘﻮﯾﺎت GSHو.GPxﻛﻤﺎ ﻟﻮﺣﻆ ارﺗﻔﺎع ﻛﺒﯿﺮ ﻓﻲ ﺟﯿﻦP53وHO-1واﻧﺨﻔﺎضﻓﻲ<br/>Hsp70وKras.ﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰذﻟﻚ،ﺗﻢ رﻓﻊ ﻧﺴﺒﺔ اﻷﻧﺴﺠﺔ اﻟﻤﯿﺘﺔ ﻓﻲ اﻟﻤﺠﻤﻮﻋﺎت اﻟﺘﻲ ﯾﺴﺒﺒﮭﺎ اﻟﻮرم وﻧﺴﺒﺔ اﻟﺨﻼﯾﺎ<br/>اﻟﺴﺮطﺎﻧﯿﺔاﻟﻤﺘﺪھﻮرةﺑﻌﺪاﻟﻌﻼج Ag: HAp-NPsﺑﺸﻜﻞﻣﻠﺤﻮظ.ﻓﯿﻤﺎﯾﺘﻌﻠﻖﺑﺴﻼﻣﺔ :Ag HAp-NPs،ﻓﻘﺪﺛﺒﺖٔ اﻧﮭﺎ<br/>ٓاﻣﻨﺔوﻏﯿﺮﺳﺎﻣﺔﻟﻠﺠﯿﻨﺎت.ﺗﻢﺗﺄﻛﯿﺪذﻟﻚﻣﻦﺧﻼل اﻟﺘﻐﯿﯿﺮات ﻏﯿﺮاﻟﻤﮭﻤﺔﻓﻲTLوTMﻓﻲﻧﺨﺎع اﻟﻌﻈﺎم ؤاﻧﺴﺠﺔاﻟﻜﺒﺪ.ﺗﻢ<br/>ﺗﺄﻛﯿﺪذﻟﻚٔای ًﺿﺎﻣﻦ ﺧﻼل اﻟﺘﻐﯿﯿﺮات ﻏﯿﺮاﻟﻤﻠﺤﻮظﺔ ﻓﻲﻣﺴﺘﻮﯾﺎت MDAوTACﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰﻧﺸﺎطSODﻓﻲاﻟﻜﺒﺪ 
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<br/><br/><br/>Text in English and abstract in Arabic & English. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Genetics
<br/><br/><label>Subjects--Index Terms: </label>Silver doped hydroxyapatite  cancer  mice  antitumor  oxidative stress and safety 
<br/><br/><label>Dewey Class. No.: </label>591.35