TY  - BOOK
AU  - Gihad Ahmed Sayed Mohammed, 
AU  - Mohsen A. Sayed
AU  - Eman Abdullah M. Ali 
TI  - Purification, characterization, and molecular studies on S- adenosyl methionine synthase in dimorphic fungi / 
U1  - 580 
PY  - 2023///
KW  - Botany and Microbiology
KW  - qrmak
KW  - Candida albicans
KW  - SAM
KW  - S-adenosyl methionine synthase
KW  - Adomet
N1  - Thesis (M.Sc.)-Cairo University, 2023.; Bibliography: pages 77-112.; Issued also as CD
N2  - Candida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing.
C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of  the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity.  Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain

ER  -