TY - GEN AU - Amr Elsayed Ahmed Yousef AU - Mervat Elsayed Mohamed , AU - Sanaa Osman Abdallah , AU - Yasser Ibrahim Hassan Alnahaas , TI - Comparision between nested pcr and quantitative rt - real time pcr in the detection of minimal residual disease in cml patients / PY - 2006/// CY - Cairo : PB - Amr Elsayed Ahmed Yousef , KW - Chronic myeloid leukemia KW - Nested RT - PCR KW - Real - Time RT - PCR N1 - Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry; Issued also as CD N2 - Real - Time RT - PCR has great advantages for estimating transcript levels in a variety of situations These include relative rapid assay times (hours) , reliability and ease of performing replicate analyses In contrast , Nested PCR is a labor procedure requiring a few days to generate useful data We compared the same samples from CML patients by both methods In this study , a total peripheral blood samples from CML patients were analyzed by RT - PCR and Nested PCR , these patients were undergoing therapy by Gleevec Our findings indicated that Nested PCR and Real Time PCR are significantly different UR - http://172.23.153.220/th.pdf ER -