TY  - GEN
AU  - Hanan Abd El Salam Mohamed Ogaly
AU  - Gamal elSayed Essawy , 
AU  - Magdy Ahmed Ghoneim , 
AU  - Mohsen Ahmed Wasfy , 
TI  - Cloning and Expression of Sheep Leptin and Studying ItsBiological Actions / 
PY  - 2006///
CY  - Cairo : 
PB  - Hanan Abd El Salam Mohamed Ogaly , 
KW  - Cloning and expression
KW  - Gen of Sheep
N1  - Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Biochemistry and Chemistry Of Nutrition; Issued also as CD
N2  - The current study was carried out to isolate the ovine leptin gene of our native sheep , to determine its nucleotide sequence , and to produce recombinant ovine leptin For this purpose the following procedures Samples from different fat depots of male sheep were collected on liquid nitrogen and used for RNA extractionA pair of primer was designed to encode the mature ovine leptin gene with additional cloning sitesThese primers and the extracted RNA together with an optimized condition were used to isolate and amplify ovine leptin cDNA through RT - PCR technique The amplified fragment (insert)
ER  -