TY - BOOK AU - Wael Abdelmonem Mohamed Hassan AU - Abdelmohsen Saber Ismail , AU - Ahmed Mahmoud Aboulenein , AU - Sayed Abdelkader Fayed , TI - Biochemical studies on the unconventional utilization of the Egyptian wild plant silybum marianum enzymes for food and medical industries / PY - 2013/// CY - Cairo : PB - Wael Abdelmonem Mohamed Hassan , KW - Milk-clotting enzymes KW - Plant rennet KW - Silybum marianum N1 - Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Biochemistry; Issued also as CD N2 - A powerful and applicable milk-clotting enzyme was extracted from the different wild Egyptian plant Silybum marianum parts. Seeds extract was the most efficient and exhibited the highest milk-clotting activity and the lowest proteolytic action. Partial purification of the enzyme seeds extract was performed by sequential and batch precipitation by different precipitating agents, i.e. ammonium sulphate, ethanol and acetone ER -